PMID: 9531538May 9, 1998Paper

Molecular genetic analysis of the heterodimeric splicing factor U2AF: the RS domain on either the large or small Drosophila subunit is dispensable in vivo

Genes & Development
D Z RudnerDonald C Rio

Abstract

The pre-mRNA splicing factor U2AF (U2 snRNP auxiliary factor) has an essential role in 3' splice site selection. U2AF binds the intron pyrimidine tract between the branchpoint and the 3' splice site and recruits U2 snRNP to the branch site at an early step in spliceosome assembly. Human U2AF is a heterodimer composed of large (hU2AF65) and small (hU2AF35) subunits. Both subunits contain a domain enriched in arginine-serine dipeptide repeats termed an RS domain. The two U2AF RS domains have been assigned essential and independent roles in spliceosome assembly in vitro-the hU2AF65 RS domain is required to target U2 snRNP to the branch site and the hU2AF35 RS domain is necessary for protein-protein interactions with constitutive and alternative splicing factors. We have investigated the functional requirements for the RS domains on the Drosophila U2AF homolog in vivo. In sharp contrast to its essential role in U2 snRNP recruitment in vitro, the RS domain on the Drosophila large subunit homolog (dU2AF50) was completely dispensable in vivo. Prompted by this unexpected result, we analyzed the RS domain on the Drosophila small subunit homolog (dU2AF38). Despite its requirement for enhancer-dependent splicing activity in vitro, the dU2...Continue Reading

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Citations

Jun 26, 2001·RNA·B R GraveleyT Maniatis
May 20, 1999·RNA·E F Modafferi, D L Black
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Arianne J MatlinChristopher W J Smith
Jan 30, 1999·Genes & Development·C BatchelderT K Blackwell
Dec 22, 2000·Genes & Development·J Zhu, A R Krainer
Jan 13, 2006·Plant Physiology·Bing-Bing Wang, Volker Brendel
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May 4, 2004·Molecular and Cellular Biology·Christopher J Webb, Jo Ann Wise
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Aug 28, 2016·Nucleic Acids Research·Jana Kralovicova, Igor Vorechovsky
Mar 31, 2018·Plant Molecular Biology·Anna LiponskaHarald Putzer
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Dec 29, 2000·The Journal of Biological Chemistry·M Gama-CarvalhoM Carmo-Fonseca
Nov 15, 2015·Genome Research·Chien-Ling LinWilliam G Fairbrother

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