PMID: 8596670Oct 1, 1995Paper

Molecular genetic detection of Bacteroides heparinolyticus in adult periodontitis

Oral Microbiology and Immunology
A AshimotoJ Slots

Abstract

Bacteroides heparinolyticus in subgingival plaque was identified using a digoxigenin-labeled whole genomic DNA probe and a polymerase chain reaction (PCR) assay based on 16S rRNA species-specific primers (5'-ATG GTG ATT CCG CAT GGT TTC TCC-3' (base position, 188-212) and 5'-CAA ACT TTC ACA GCT GAC TTA AGC-3' (592-615)). Subgingival specimens obtained by paper points from 3 deep periodontal pockets in each of 113 adults were examined. The DNA probe reacted with all pure isolates tested of B. heparinolyticus and did not react with other oral species tested; the probe showed positive reactions in 74.3% of the patient samples examined. The PCR primers produced the 428 bp species specific amplification product in all B. heparinolyticus test strains and did not reveal detectable amplicons with strains of other subgingival species. The PCR method detected 50 B. heparinolyticus cells dispersed in subgingival plaque. PCR only revealed B. heparinolyticus in 6.2% of the patient samples studied. The higher level of positive specimens with the DNA probe was probably due to false-positive reactions from cross-hybridization with unknown subgingival species. This study suggests that the PCR method amplifying specific 16S rRNA sequences represe...Continue Reading

References

Jan 1, 1990·Archives of Oral Biology·E D SavittW J Peros
May 1, 1988·Journal of Clinical Microbiology·T NakamuraS Fujimura
Dec 1, 1983·Applied and Environmental Microbiology·H TaniguchiT Nakamura
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J SlotsC Chen
Jun 1, 1994·Oral Microbiology and Immunology·R F LotufoJ Slots
Feb 1, 1994·Journal of Bacteriology·B J PasterG J Fraser
Feb 1, 1995·Periodontology 2000·J J Zambon, V I Haraszthy
Jun 1, 1994·Periodontology 2000·W E Moore, L V Moore
Jun 1, 1994·Periodontology 2000·A D Haffajee, S S Socransky

❮ Previous
Next ❯

Citations

Jul 10, 2001·Journal of Clinical Periodontology·I B DarbyD F Kinane
Nov 1, 1996·Annals of Periodontology·G C Armitage
Jan 5, 2006·Oral Microbiology and Immunology·G Dahlén, A Leonhardt
Jun 1, 1996·Journal of Clinical Periodontology·B von Troil-LindénS Asikainen
Dec 10, 2020·Journal of Medical Microbiology·Ioannis FragkioudakisDanae Anastasia Apatzidou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.