Molecular genetic diversity and characterization of conjugation genes in the fish parasite Ichthyophthirius multifiliis

Molecular Phylogenetics and Evolution
Elisabeth MacCollWei-Jen Chang

Abstract

Ichthyophthirius multifiliis is the etiologic agent of "white spot", a commercially important disease of freshwater fish. As a parasitic ciliate, I. multifiliis infects numerous host species across a broad geographic range. Although Ichthyophthirius outbreaks are difficult to control, recent sequencing of the I. multifiliis genome has revealed a number of potential metabolic pathways for therapeutic intervention, along with likely vaccine targets for disease prevention. Nonetheless, major gaps exist in our understanding of both the life cycle and population structure of I. multifiliis in the wild. For example, conjugation has never been described in this species, and it is unclear whether I. multifiliis undergoes sexual reproduction, despite the presence of a germline micronucleus. In addition, no good methods exist to distinguish strains, leaving phylogenetic relationships between geographic isolates completely unresolved. Here, we compared nucleotide sequences of SSUrDNA, mitochondrial NADH dehydrogenase subunit I and cox-1 genes, and 14 somatic SNP sites from nine I. multifiliis isolates obtained from four different states in the US since 1995. The mitochondrial sequences effectively distinguished the isolates from one anoth...Continue Reading

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Citations

Jan 27, 2017·PeerJ·Erhan AslanMuhittin Arslanyolu
Feb 25, 2017·Frontiers in Microbiology·Kassandra E ZailaWei-Jen Chang
Aug 14, 2020·Genome Biology and Evolution·Yi FengLaura F Landweber

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