PMID: 9445043Jan 28, 1998Paper

Molecular genetic study of the interaction of Sindbis virus E2 with Ross River virus E1 for virus budding

Journal of Virology
J YaoJ H Strauss

Abstract

Glycoprotein PE2 of Sindbis virus will form a heterodimer with glycoprotein E1 of Ross River virus that is cleaved to an E2/E1 heterodimer and transported to the cell plasma membrane, but this chimeric heterodimer fails to interact with Sindbis virus nucleocapsids, and very little budding to produce mature virus occurs upon infection with chimeric viruses. We have isolated in both Sindbis virus E2 and in Ross River virus E1 a series of suppressing mutations that adapt these two proteins to one another and allow increased levels of chimeric virus production. Two adaptive E1 changes in an ectodomain immediately adjacent to the membrane anchor and five adaptive E2 changes in a 12-residue ectodomain centered on Asp-242 have been identified. One change in Ross River virus E1 (Gln-411-->Leu) and one change in Sindbis virus E2 (Asp-248-->Tyr) were investigated in detail. Each change individually leads to about a 10-fold increase in virus production, and combined the two changes lead to a 100-fold increase in virus. During passage of a chimeric virus containing Ross River virus E1 and Sindbis virus E2, the E2 change was first selected, followed by the E1 change. Heterodimers containing these two adaptive mutations have a demonstrably i...Continue Reading

References

Nov 1, 1978·Journal of Virology·C H von Bonsdorff, S C Harrison
May 1, 1974·Journal of Virology·J S PierceJ H Strauss
Feb 1, 1995·The Journal of Cell Biology·B U BarthH Garoff
Sep 1, 1994·Microbiological Reviews·J H Strauss, E G Strauss
Feb 18, 1994·Journal of Molecular Biology·C Vénien-Bryan, S D Fuller
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A M ParedesB V Prasad
Sep 1, 1995·Trends in Microbiology·J H StraussR J Kuhn

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Citations

Mar 26, 2010·Viruses·Margaret KielianMaofu Liao
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·H GaroffD J Opstelten
Nov 30, 2004·Virus Research·Henrik GaroffR Holland Cheng

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