Molecular Genetics of Lidocaine-Containing Cardioplegia in the Human Heart During Cardiac Surgery

The Annals of Thoracic Surgery
Mahyar HeydarpourJochen D Muehlschlegel

Abstract

During cardiac surgery with cardiopulmonary bypass, delivery of cardioplegia solution to achieve electromechanical cardiac quiescence is obligatory. The addition of lidocaine to cardioplegia has advantages, although its consequences at a molecular level remain unclear. We performed whole-genome RNA sequencing of the human left ventricular (LV) myocardium to elucidate the differences between whole-blood (WB) cardioplegia with and without addition of lidocaine (LC) on gene expression. We prospectively enrolled 130 patients undergoing aortic valve replacement surgery. Patients received high-potassium blood cardioplegia either with (n = 37) or without (n = 93) lidocaine. The LV apex was biopsied at baseline, and after an average of 74 minutes of cold cardioplegic arrest. We performed differential gene expression analysis for 18,258 genes between these 2 groups. Clinical and demographic variables were adjusted in the model. Gene ontology (GO) and network enrichment analysis of the retained genes were performed using g:Profiler and Cytoscape. A total of 1,298 genes were differentially expressed between cardioplegic treatments. Compared with the WB group, genes upregulated in the LC group were identified by network enrichment to play ...Continue Reading

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Citations

Mar 1, 2020·The Journal of Thoracic and Cardiovascular Surgery·Marien LenoirPhilippe Demers
Nov 11, 2019·The Journal of Thoracic and Cardiovascular Surgery·Tomasz A TimekCharles L Willekes

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