Molecular genotyping of Candida parapsilosis group I clinical isolates by analysis of polymorphic microsatellite markers

Journal of Clinical Microbiology
Brent A LaskerT J Lott

Abstract

Candida parapsilosis, a pathogenic yeast, is composed of three newly designated genomic species that are physiologically and morphologically indistinguishable. Nosocomial infections caused by group I C. parapsilosis are often associated with the breakdown of infection control practices and the contamination of medical devices, solutions, and indwelling catheters. Due to the low levels of nucleotide sequence variation that are observed, an investigation of the size polymorphisms in loci harboring microsatellite repeat sequences was applied for the typing of C. parapsilosis group I isolates. PCR primer sets that flank the microsatellite repeats for seven loci were designed. Following amplification by PCR, the size of each amplification product was determined automatically by capillary electrophoresis. A total of 42 C. parapsilosis group I isolates were typed by microsatellite analysis, and their profiles were compared to the hybridization profiles obtained by use of the Cp3-13 DNA probe. A high degree of discrimination (discriminatory power = 0.971) was observed by microsatellite analysis. The number of different alleles per locus ranged from 14 for locus B to 5 for locus C. Microsatellite analysis detected 30 different microsate...Continue Reading

References

Mar 11, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J J Weems
Nov 1, 1992·Genetics·R M HardingJ B Clegg
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J F Dallas
Jan 25, 1992·Nucleic Acids Research·C Schlötterer, D Tautz
Sep 1, 1990·Journal of Clinical Microbiology·P R Hunter
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W R Jarvis
Jul 1, 1995·Journal of Clinical Microbiology·D LinP F Lehmann
Jan 1, 1995·Diagnostic Microbiology and Infectious Disease·M A PfallerR J Hollis
Sep 1, 1994·Journal of Clinical Microbiology·L J StrausbaughR Tjoelker
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·A Di RienzoN B Freimer
May 1, 1993·Current Genetics·T J LottB A Lasker
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·G OrtíJ C Avise
Feb 12, 1998·Journal of Clinical Microbiology·B Roy, S A Meyer
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·S KruglyakC F Aquadro
Sep 7, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M B EdmondR P Wenzel
Oct 19, 1999·Journal of Clinical Microbiology·F De BernardisA Cassone
Feb 15, 2000·Research in Microbiology·G F RichardB Dujon
Sep 27, 2000·The Journal of Biological Chemistry·J P Jakupciak, R D Wells
Feb 7, 2001·Journal of Clinical Microbiology·C HennequinB Dujon
Feb 7, 2001·Journal of Clinical Microbiology·L EngerD R Soll
Oct 30, 2001·Journal of Clinical Microbiology·F BotterelS Bretagne
Jun 29, 2002·Journal of Clinical Microbiology·Antonella LupettiSonia Senesi
Feb 8, 2003·Journal of Clinical Microbiology·Paula SampaioCélia Pais
Aug 28, 2003·Current Opinion in Microbiology·John W Taylor, Matthew C Fisher
Mar 17, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ruth E FundygaTimothy J Lott
Apr 9, 2004·Journal of Clinical Microbiology·Brent A Lasker, Yuping Ran
Apr 16, 2004·Clinical Microbiology Reviews·Erna M Kojic, Rabih O Darouiche
Jun 23, 2004·Emerging Infectious Diseases·Duncan M KuhnMahmoud A Ghannoum
Jul 13, 2004·Diagnostic Microbiology and Infectious Disease·Lucía García San MiguelSantiago Moreno
Sep 11, 2004·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Brunella PosteraroGiovanni Fadda

❮ Previous
Next ❯

Citations

Aug 8, 2009·Current Genetics·Jozef NosekLubomir Tomaska
May 31, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G PulcranoM R Catania
Jan 24, 2013·The Pediatric Infectious Disease Journal·Mohan PammiJoseph M Bliss
Jan 13, 2010·Clinical Microbiology Reviews·Geraldine Butler
Feb 22, 2011·Eukaryotic Cell·Sixiang SaiGeraldine Butler
Jun 21, 2013·Journal of Clinical Microbiology·Tâmara Heloísa Rocha PrandiniEduardo Bagagli
Sep 15, 2007·Journal of Clinical Microbiology·Frédéric GrenouilletRenaud Piarroux
Mar 12, 2010·Journal of Clinical Microbiology·Raquel SabinoCélia Pais
May 9, 2012·PloS One·Alessandro RiccombeniGeraldine Butler
Oct 14, 2009·Critical Reviews in Microbiology·Eveline C van AsbeckDavid A Stevens
Jun 26, 2013·Revista iberoamericana de micología·Laura Judith Marcos-ZambranoJesús Guinea
Nov 14, 2012·Revista iberoamericana de micología·Luciana da Silva RuizClaudete Rodrigues Paula
May 17, 2011·International Journal of Medical Microbiology : IJMM·M del Pilar VercherLucas del Castillo Agudo
Apr 1, 2008·Journal of Microbiological Methods·S Arunmozhi BalajeeCorné H W Klaassen
Aug 21, 2010·Letters in Applied Microbiology·C D VieiraL de Macêdo Farias
Jun 14, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R SabinoC Pais
Dec 18, 2013·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Kelsey OyongLaurene Mascola
Jul 12, 2013·BioMed Research International·Claudia Spampinato, Darío Leonardi
Jul 10, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Errol ReissShawn R Lockhart
Jan 17, 2015·Trends in Parasitology·V RougeronA-L Bañuls
Feb 23, 2017·Memórias do Instituto Oswaldo Cruz·Leonardo Silva BarbedoRosely Maria Zancopé-Oliveira
Apr 7, 2012·Genetics and Molecular Biology·Adolfo Jose MotaFrancisco G Nobrega
Jul 9, 2008·Scandinavian Journal of Infectious Diseases·Eveline C Van AsbeckUNKNOWN Candida Parapsilosis Global Epidemiology Group
Jul 12, 2018·Mycopathologia·Sourour NejiAli Ayadi
May 12, 2017·Clinical Microbiology Reviews·Alexandre AlanioStéphane Bretagne
Sep 16, 2008·Analytical and Bioanalytical Chemistry·Ingolf Schimke
Nov 22, 2008·Scandinavian Journal of Infectious Diseases·Anna Brillowska-DabrowskaM C Arendrup
Nov 18, 2017·Mycopathologia·Marie Desnos-OllivierUNKNOWN Uruguayan Invasive Fungal Infection Network
May 1, 2018·Current Medical Mycology·Hamid BadaliSadegh Khodavaisy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.