Molecular Genotyping of Shigella sonnei Strains Isolated From Children With Bloody Diarrhea Using Pulsed Field Gel Electrophoresis on the Total Genome and PCR-RFLP of IpaH and IpaBCD Genes

Jundishapur Journal of Microbiology
Shohreh FarshadMarziyeh Hosseini

Abstract

Identification, understanding of antibiotic sensitivity patterns and molecular characterization of genetic elements of Shigella species are important because of both epidemiological and clinical indications in developing countries. The aim of this study was to analyze molecular epidemiology of Shigella isolates recovered from children with diarrhea in Shiraz (Southern Iran), using IpaH and IpaBCD PCR-restriction fragment length polymorphism (RFLP), and to determine pulsed field gel electrophoresis (PFGE) patterns of total DNA of the S. sonnei isolates to find the clonality among these strains. A total of 82 clinical strains of Shigella spp., S. sonnei (n = 61), S. flexneri (n = 16), Shigella boydii (n = 3) and S. dysenteriae (n = 2) isolated from the stool samples of 719 patients, aged two months to 14 years, with positive occult blood (OB) test were characterized based on their IpaH and IpaBCD genes PCR-RFLP patterns. Genomic DNAs of S. sonnei strains were analyzed by PFGE. All Shigella isolates were positive for both invasive genes and showed homogeneous profiles for such genes except for two S. sonnei strains, which had IpaH bands with different sizes and PCR-RFLP profiles. Forty palsotypes were determined among the 41 S. so...Continue Reading

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Citations

Nov 14, 2019·International Journal of Environmental Research and Public Health·Chidozie Declan Iwu, Anthony Ifeanyi Okoh
Aug 7, 2017·Osong Public Health and Research Perspectives·Sajad YaghoubiMahmood Mahmoudi

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Methods Mentioned

BETA
genotyping
electrophoresis
PCR
ribotyping

Software Mentioned

Photo Capt Mw
Numerical Taxonomy and Multivariate Analysis System
NTSYS
- PC

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