Molecular gut content analysis of different spider body parts

PloS One
Nuria Macías-HernándezJames D Harwood

Abstract

Molecular gut-content analysis has revolutionized the study of food webs and feeding interactions, allowing the detection of prey DNA within the gut of many organisms. However, successful prey detection is a challenging procedure in which many factors affect every step, starting from the DNA extraction process. Spiders are liquid feeders with branched gut diverticula extending into their legs and throughout the prosoma, thus digestion takes places in different parts of the body and simple gut dissection is not possible. In this study, we investigated differences in prey detectability in DNA extracts from different parts of the spider´s body: legs, prosoma and opisthosoma, using prey-specific PCR and metabarcoding approaches. We performed feeding trials with the woodlouse hunter spider Dysdera verneaui Simon, 1883 (Dysderidae) to estimate the time at which prey DNA is detectable within the predator after feeding. Although we found that all parts of the spider body are suitable for gut-content analysis when using prey-specific PCR approach, results based on metabarcoding suggested the opisthosoma is optimal for detection of predation in spiders because it contained the highest concentration of prey DNA for longer post feeding per...Continue Reading

Associated Datasets

Jan 28, 2017·Owen S. WangensteenNuria Macias-Hernandez

References

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Citations

Jul 25, 2019·Molecular Ecology Resources·Luís P da SilvaPedro Beja
Nov 12, 2019·Ecological Applications : a Publication of the Ecological Society of America·Xavier TuronOwen Simon Wangensteen
Jul 21, 2020·Molecular Ecology Resources·Stella F Uiterwaal, John P DeLong
Jun 25, 2020·Ecological Applications : a Publication of the Ecological Society of America·Stacy M PhilpottHeidi Liere
Jun 20, 2019·Scientific Reports·Cristian Cuevas-CaballéMarta Álvarez-Presas
Jun 19, 2021·Ecology and Evolution·Diego Sierra RamírezCarlos Fernando Prada Quiroga
Aug 12, 2021·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Luiza Helena GremskiSilvio Sanches Veiga
Sep 4, 2021·Cladistics : the International Journal of the Willi Hennig Society·Miquel A Arnedo, Gustavo Hormiga
Sep 21, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Tanya StrydomTimothée Poisot

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
dissection
PCR
scraping
electrophoresis
PCRs
Assay
Illumina sequencing
dissect

Software Mentioned

vsearch
SAS
Geneious
illuminapairedend
ngsfilter
ecoPCR
obiuniq
MUSCLE
CROP
FastQC

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