PMID: 8972876Dec 1, 1996Paper

Molecular haplotyping of genetic markers 10 kb apart by allele-specific long-range PCR

Nucleic Acids Research
S Michalatos-BeloinG Ruano

Abstract

Haplotypes, combinations of polymorphic markers in a chromosome, are critical for genome diversity research. However, their utility in population samplings is compromised by uncertain linkage phase determinations from unrelated individuals. Molecular haplotyping accomplishes direct phase determination by generation of hemizygous templates from diploid genomic samples. We report molecular haplotyping by allele-specific long-range PCR of two markers 9.5 kb apart at the CD4 locus: a bi-allelic Alu deletion and a multi-allelic repeat. We verified CD4 molecular haplotypes by classical Mendelian analysis. Molecular haplotyping should prove useful in mapping disease genes and in establishing founder effects.

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Citations

Mar 29, 2002·Genetic Epidemiology·Katrina A B Goddard, Ellen M Wijsman
Jan 1, 2003·Genetic Epidemiology·Shuang WangHongyu Zhao
Apr 13, 2011·Cellular and Molecular Neurobiology·Halimeh Rezaei, Sadeq Vallian
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Dec 21, 2010·Nature Biotechnology·H Christina FanStephen R Quake
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Dec 18, 2012·Genome Biology and Evolution·Zsófia BánlakiMárton Doleschall
Dec 13, 2006·Nucleic Acids Research·Bernard A KonfortovPaul H Dear
Jun 20, 2008·Nucleic Acids Research·Daniel J TurnerMatthew E Hurles
Jul 26, 2002·Nucleic Acids Research·Ester InbarAriel Darvasi
Oct 14, 2005·Nucleic Acids Research·Sarah J LindsayMatthew E Hurles
May 21, 2009·Annual Review of Genomics and Human Genetics·Jiannis Ragoussis
Jan 22, 2005·Annual Review of Medicine·Dana C Crawford, Deborah A Nickerson
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Aug 8, 2002·Pharmacogenomics·Ann E Ferentz
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