Molecular hazard identification of non-O157 Shiga toxin-producing Escherichia coli (STEC)

PloS One
Eelco FranzHenk J M Aarts

Abstract

The complexity regarding Shiga toxin-producing Escherichia coli (STEC) in food safety enforcement as well as clinical care primarily relates to the current inability of an accurate risk assessment of individual strains due to the large variety in serotype and genetic content associated with (severe) disease. In order to classify the clinical and/or epidemic potential of a STEC isolate at an early stage it is crucial to identify virulence characteristics of putative pathogens from genomic information, which is referred to as 'predictive hazard identification'. This study aimed at identifying associations between virulence factors, phylogenetic groups, isolation sources and seropathotypes. Most non-O157 STEC in the Netherlands belong to phylogroup B1 and are characterized by the presence of ehxA, iha and stx2, but absence of eae. The large variability in the number of virulence factors present among serogroups and seropathotypes demonstrated that this was merely indicative for the virulence potential. While all the virulence gene associations have been worked out, it appeared that there is no specific pattern that would unambiguously enable hazard identification for an STEC strain. However, the strong correlations between virulen...Continue Reading

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Citations

Jan 20, 2017·Foodborne Pathogens and Disease·Victoria BrusaLucía Galli
Aug 26, 2016·Clinical Microbiology Reviews·J RonholmFranco Pagotto
Mar 28, 2019·Journal of Food Protection·National Advisory Committee On Microbiological Criteria For Foods
Jan 5, 2021·Foodborne Pathogens and Disease·Siobhán C McCarthyGeraldine Duffy
Feb 1, 2021·Water Research·Lapo Mughini-GrasAna Maria de Roda Husman

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Methods Mentioned

BETA
PCR
electrophoresis
environmental stress

Software Mentioned

SPSS Statistics
BioNumerics

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