Molecular identification and antifungal susceptibility of Curvularia australiensis, C. hawaiiensis and C. spicifera isolated from human eye infections

Mycoses
Krisztina KrizsánTamás Papp

Abstract

A reliable identification method was developed for three closely related Curvularia species, which are frequently isolated from human keratomycoses. Since the traditionally used morphological method and the increasingly used internal transcribed spacer (ITS)-based molecular method proved to be insufficient to discern C. australiensis, C. hawaiiensis and C. spicifera, other molecular targets, such as β-tubulin, translation elongation factor 1-α and the nuclear ribosomal intergenic spacer (IGS), were tested. Among them, the use of the highly divergent IGS sequence is suggested and the species-specific discriminating characters were determined in appropriate reference strains. It was also concluded that C. hawaiiensis and C. spicifera can be predominantly isolated from eye infections among the three species. The in vitro antifungal susceptibility of 10 currently used antifungal agents against 32 Curvularia isolates was also investigated. MICs were determined in each case. Isolates of C. spicifera proved to be less susceptible to the tested antifungals than those of C. hawaiiensis, which underline the importance of the correct identification of these species.

References

Aug 1, 1986·Journal of Clinical Microbiology·M R McGinnisR E Winn
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·T J Walsh, A H Groll
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Oct 11, 2001·Journal of Perinatology : Official Journal of the California Perinatal Association·M L MooreT S Russell
Feb 13, 2003·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Edward J ShinAnil K Lalwani
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Oct 15, 2003·Clinical Microbiology Reviews·Philip A Thomas
Dec 6, 2003·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Marcelo J FilizzolaShariq J Rauf
Jan 17, 2004·Medical Mycology·A J BavaM Sandoval
Jul 3, 2004·Current Opinion in Ophthalmology·M Srinivasan
Dec 14, 2004·Journal of Cutaneous Medicine and Surgery·Robert C Hayes, Anne Curtis
Jul 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ari Löytynoja, Nick Goldman
Sep 29, 2005·Journal of Cutaneous Medicine and Surgery·Donna BiluMargaret Moresi
Apr 20, 2006·The Journal of Infection·P ToulM Gari-Toussaint
May 10, 2007·Mycologia·Manfred Binder, David S Hibbett
Aug 22, 2007·Medical Mycology·Katsuji TaguchiKei Takahashi
Apr 10, 2008·Molecular Biology and Evolution·David Posada
Apr 11, 2008·Mycoses·P K ShuklaG B S Keshava
Apr 17, 2008·Medical Mycology·Hiromi KobayashiShinichiro Hayashi
Jul 8, 2008·Indian Journal of Pathology & Microbiology·Jagdish ChanderAntariksh Deep
Jul 16, 2008·Mycopathologia·Gulnaz BashirAzra Rizvi
Jul 16, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·George M Viola, Richard Sutton
Feb 27, 2013·Mycoses·Katihuska ParedesJosep Guarro

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Citations

Jul 12, 2018·Mycoses·Shahram MahmoudiAli Banafsheafshan
Jan 29, 2018·Mycopathologia·Mónika HomaLászló Kredics
Jan 4, 2019·Journal of Clinical Microbiology·Iker Falces-RomeroJulio García-Rodríguez
Jun 3, 2021·Clinical Microbiology Reviews·Sixto M LealPeter H Gilligan
Sep 18, 2018·ACS Omega·Josefa RosellóM Pilar Santamarina

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