Molecular identification and characterization of a gene associated with the onset of tapping panel dryness (TPD) syndrome in rubber tree (Hevea brasiliensis Muell.) by mRNA differential display.

Molecular Biotechnology
P VenkatachalamKashchandra Raghothama

Abstract

In rubber tree (Hevea brasiliensis), tapping panel dryness (TPD) syndrome is considered as a complex physiological disorder which affects latex biosynthesis. To identify differentially expressed genes between healthy and TPD-affected trees, mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) analysis was performed. We isolated 10 differentially expressed cDNA fragments of which one cDNA encoding a putative TOM20 like protein was identified. The cDNA (1,024 bp), corresponding to the HbTOM20 gene (H evea b rasiliensis Translocase of the Outer Mitochondrial Membrane), contained an open reading frame to code for 202 amino acid protein with a theoretical pI value of 9.5 and the calculated protein M (W) was 23.5 kDa. The predicted amino acid sequence contained conserved domains of TOM20 like proteins in the N-terminal. The protein HbTOM20 has 32% and 27% similarity to Populus TOM20 and Solanum TOM20, respectively. Both semi-quantitative RT-PCR and Northern blot results revealed that the HbTOM20 expression was significantly down-regulated in TPD-affected trees compared to healthy one. Accumulation of HbTOM20 mRNA transcripts was significantly higher in the bark tissues collected from healthy region tha...Continue Reading

References

May 1, 1989·Pflügers Archiv : European journal of physiology·J EhrenfeldB J Harvey
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jun 1, 1996·The Plant Journal : for Cell and Molecular Biology·L Heins, U K Schmitz
Apr 1, 1997·Journal of Bioenergetics and Biomembranes·N ZamzamiG Kroemer
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·L J SweetloveA H Millar
Mar 26, 2003·Tree Physiology·Kyung-Hwan HanK. S. Chow
Sep 6, 2005·Analytical Biochemistry·Li-ji Zhu, Scott W Altmann
Sep 1, 2006·Plant Science : an International Journal of Experimental Plant Biology·Gisele A M TorresChristine Lelandais-Brière

❮ Previous
Next ❯

Citations

Jan 18, 2013·BMC Genomics·Cuifang DuanPascal Montoro
Jun 29, 2014·Journal of Plant Physiology·Manassawe LertpanyasampathaJarunya Narangajavana
Nov 15, 2011·Plant Physiology and Biochemistry : PPB·Shi-Qing PengShou-Cai Chen
May 18, 2010·Plant Physiology and Biochemistry : PPB·Jiahong ZhuZhili Zhang
Mar 24, 2016·Genetics and Molecular Biology·Zi-Ping YangShi-Qing Peng
Feb 13, 2014·Anais Da Academia Brasileira De Ciências·Caio D GuzzoJosé V F Martins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.