Molecular identification and characterization of rhodaneses from the insect herbivore Pieris rapae

Scientific Reports
Anna-Maria SteinerUte Wittstock

Abstract

The association of cabbage white butterflies (Pieris spec., Lepidoptera: Pieridae) with their glucosinolate-containing host plants represents a well-investigated example of the sequential evolution of plant defenses and insect herbivore counteradaptations. The defensive potential of glucosinolates, a group of amino acid-derived thioglucosides present in plants of the Brassicales order, arises mainly from their rapid breakdown upon tissue disruption resulting in formation of toxic isothiocyanates. Larvae of P. rapae are able to feed exclusively on glucosinolate-containing plants due to expression of a nitrile-specifier protein in their gut which redirects glucosinolate breakdown to the formation of nitriles. The release of equimolar amounts of cyanide upon further metabolism of the benzylglucosinolate-derived nitrile suggests that the larvae are also equipped with efficient means of cyanide detoxification such as β-cyanoalanine synthases or rhodaneses. While insect β-cyanoalanine synthases have recently been identified at the molecular level, no sequence information was available of characterized insect rhodaneses. Here, we identify and characterize two single-domain rhodaneses from P. rapae, PrTST1 and PrTST2. The enzymes diffe...Continue Reading

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Citations

Sep 21, 2018·Journal of Chemical Ecology·Adam M Rork, Tanya Renner
Jan 8, 2022·Annual Review of Entomology·Franziska Beran, Georg Petschenka

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Datasets Mentioned

BETA
XP021932810
MH036751
MH036752

Methods Mentioned

BETA
RNAseq
PCR
Illumina sequencing
Protein Assay
Whole Genome Sequencing

Software Mentioned

OriginPro
MUSCLE
PhyML
Geneious
tBLASTn
InterProScan
BLAST2GO PRO
Gene
Ontology
CLC Genomics Workbench

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