Abstract
In maize, a small multigene family encodes the cytosolic isoforms of glutamine synthetase (GS), and five cDNAs, designated pGS1a, pGS1b, pGS1c, pGS1d, and pGS1e, have been cloned (Sakakibara, H., Kawabata, S., Takahashi, H., Hase, T., and Sugiyama, T. (1992) Plant Cell Physiol. 33, 49-58; Li, M., Villemur, R., Hussey, P. J., Silflow, C. D., Gantt, J. S., and Snustad, D. P. (1993) Plant Mol. Biol. 23, 401-407). This report describes the identification and enzymatic characterization of the cytosolic isoforms of GS in maize roots, namely GS1 and GSr. The purified isoforms, as well as recombinant enzymes that had been overexpressed in Escherichia coli, were analyzed by capillary liquid chromatography/electrospray ionization-mass spectrometry, and GS1 and GSr were identified as the products of the GS1a/GS1b and GS1c/GS1d genes, respectively. Upon the addition of ammonia to the culture medium, significant amounts of GSr accumulated and a preferential increase in GS synthetase activity, as compared to GS transferase activity, was found in the root extract. Assays with the purified recombinant enzymes confirmed that the specific biosynthetic and synthetase activities of GSr were 1.6-fold higher than those of GS1. Marked differences in ...Continue Reading
References
May 28, 1979·Biochemical and Biophysical Research Communications·A F MannG R Stewart
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1992·Journal of General Microbiology·G MancoM Iaccarino
Nov 1, 1991·Molecular & General Genetics : MGG·T K Peterman, H M Goodman
Apr 1, 1990·Plant Molecular Biology·J M CockJ V Cullimore
Jul 1, 1988·Analytical Biochemistry·G J WadsworthJ G Scandalios
Jun 1, 1968·Biochemistry·B M Shapiro, A Ginsburg
Oct 1, 1967·Archives of Biochemistry and Biophysics·C A Woolfolk, E R Stadtman
Aug 15, 1970·Nature·U K Laemmli
Sep 1, 1993·Plant Physiology·A C StanfordJ V Cullimore
Oct 1, 1993·Plant Molecular Biology·M G LiD P Snustad
Apr 1, 1993·Analytical Biochemistry·H MurataY Shimonishi
Oct 1, 1980·Plant Physiology·B Hirel, P Gadal
Jul 1, 1989·Plant Physiology·L P Vézina, J R Langlois
Dec 1, 1991·Plant Physiology·T HaseY Mukohata
Nov 1, 1989·Planta·M J Bennett, J V Cullimore
May 1, 1989·Plant Molecular Biology·M J BennettJ V Cullimore
Dec 1, 1980·Planta·J V Cullimore, A P Sims
Citations
May 11, 2006·Planta·Marco BettiAntonio J Márquez
Oct 20, 2012·Molecular Genetics and Genomics : MGG·Jose Luis OrtegaChampa Sengupta-Gopalan
Oct 21, 2010·Journal of Experimental Botany·Jérémy LothierCéline Masclaux-Daubresse
Feb 10, 1999·Plant Physiology·T MatsumuraT Hase
May 15, 2001·Plant Physiology·J L OrtegaC Sengupta-Gopalan
Dec 2, 2006·The Plant Cell·Antoine MartinBertrand Hirel
Feb 4, 2014·Journal of Plant Physiology·Igor SetiénMaría Begoña González-Moro
Aug 5, 2014·Molecular Biology Reports·Wei ZhaoQuan-Hong Yao
Nov 19, 2009·The Protein Journal·Sudesh Kumar Yadav
Nov 26, 2010·FEBS Letters·Ryoung ShinDaniel P Schachtman
Mar 15, 2013·Journal of Plant Physiology·Igor SetiénJosé María Estavillo
Jul 6, 2010·Protein Expression and Purification·J M van RooyenB T Sewell
May 9, 2009·The New Phytologist·Stéphanie M Bernard, Dimah Z Habash
Apr 1, 2010·Journal of the Science of Food and Agriculture·Feifei SunXilin Hou
Aug 22, 2009·Pest Management Science·Andrea OcchipintiGiuseppe Forlani
Aug 4, 2006·Journal of Structural Biology·Oscar LlorcaJosé M Valpuesta
Apr 19, 2015·BMC Plant Biology·Bhakti Prinsi, Luca Espen
Jul 13, 2016·Journal of Phycology·Diana L ReineckeSammy Boussiba
Jul 16, 2010·Microbiological Research·H Benan DincturkHande Akce
Mar 26, 2002·Journal of Experimental Botany·María Fernanda SuárezFrancisco M Cánovas
Jul 26, 2000·FEBS Letters·C Avila SáezF M Cánovas
Dec 3, 2004·Plant & Cell Physiology·Keiki IshiyamaHideki Takahashi
Jan 17, 2019·Plant & Cell Physiology·Yuanyuan JiYangdou Wei
Jul 11, 2006·The Journal of Biological Chemistry·Hideaki UnnoMasami Kusunoki
Aug 3, 2002·Plant & Cell Physiology·Fernando de la TorreFrancisco M Cánovas
Feb 6, 2004·The Journal of Biological Chemistry·Keiki IshiyamaHideki Takahashi
Jan 27, 2018·Plant & Cell Physiology·Noriyuki KonishiSoichi Kojima
Dec 13, 2005·Plant & Cell Physiology·Keiki IshiyamaHideki Takahashi
Oct 26, 2021·Frontiers in Plant Science·Ana Zangirolame Gonçalves, Helenice Mercier