Molecular identification and localization of P2X receptors in the rat lens

Experimental Eye Research
Haruna Suzuki-KerrJulie Lim

Abstract

There is growing evidence suggesting that purine-mediated signaling pathways play important roles in the ocular lens. We have previously reported the expression of P2Y receptors in the lens; and in this present study, we show that P2X receptors are also expressed. Transcripts for P2X1-7 receptors were detected in lens fiber cells using the reverse-transcription polymerase chain reaction (RT-PCR). Western blot analysis confirmed the expression of all P2X receptor subtypes at the protein level. Immunohistochemistry revealed P2X1 and P2X7 expression in the cytoplasm of cortical fiber cells. P2X2 expression was confined to the apical-apical interface between epithelial and fiber cells with little expression in cortical fiber cells. In contrast, P2X3, P2X4, P2X5 and P2X6 were expressed throughout the lens extending from the outer cortex through to the core. In the outer cortex, immunolocalisation of these P2X receptors was predominantly cytoplasmic. However, deeper into the lens, P2X receptor immunolabeling became more membranous, indicating the recruitment of P2X receptors from cytoplasmic vesicles into the membranes of mature fiber cells in the lens core. Western blotting confirmed regional differences in P2X receptor expression. ...Continue Reading

References

Jun 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S Bassnett, D C Beebe
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·F SotoW Stühmer
Nov 19, 1997·Neuropharmacology·G ColloG Buell
Jun 17, 1998·The Journal of Biological Chemistry·A NewboltF Rassendren
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·C H MitchellM M Civan
May 4, 1999·Ophthalmic Research·R Dahm
Apr 14, 2000·Brain Research. Molecular Brain Research·T H Wheeler-SchillingE Guenther
Aug 3, 2001·Brain Research. Molecular Brain Research·T H Wheeler-SchillingR Jabs
May 11, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Angelina N Ramirez, Diana L Kunze
Sep 25, 2002·Physiological Reviews·R Alan North
Oct 22, 2002·Experimental Eye Research·George Duncan, David J Collison
May 23, 2003·Molecular Pharmacology·Xuenong BoR Alan North
Aug 26, 2003·Microscopy Research and Technique·Marc D JacobsChristian Soeller
Sep 2, 2003·Biochimica Et Biophysica Acta·Erik M Schwiebert, Akos Zsembery
Nov 5, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Nasser A Farahbakhsh
Jan 8, 2004·European Journal of Pharmacology·Peter Illes, J Alexandre Ribeiro
Sep 28, 2004·Journal of Cellular Biochemistry·Veronica E KlepeisVickery Trinkaus-Randall
Dec 30, 2004·Investigative Ophthalmology & Visual Science·Priya GomesBernard Himpens
Jan 20, 2005·The Journal of Biological Chemistry·Nelson P BarreraJ Michael Edwardson
Jan 22, 2005·Clinical and Experimental Pharmacology & Physiology·Paul J DonaldsonMarc D Jacobs
Mar 1, 2005·The Journal of Comparative Neurology·Lin-Chien HuangGary D Housley
May 18, 2005·Biochemical and Biophysical Research Communications·Boonlert CheewatrakoolpongScott Greenfeder
Jul 27, 2005·Investigative Ophthalmology & Visual Science·Julie LimPaul J Donaldson
Jan 25, 2006·Investigative Ophthalmology & Visual Science·Kaa-Sandra N CheePaul J Donaldson
Jun 15, 2007·The Journal of Membrane Biology·Richard T MathiasPaul Donaldson
Aug 29, 2007·Investigative Ophthalmology & Visual Science·Monica M Lurtz, Charles F Louis
Oct 27, 2007·Investigative Ophthalmology & Visual Science·Julie LimPaul J Donaldson

❮ Previous
Next ❯

Citations

Apr 14, 2011·Purinergic Signalling·Geoffrey Burnstock
Dec 15, 2015·Pulmonary Pharmacology & Therapeutics·Mario CazzolaMaria Gabriella Matera
Jun 30, 2009·Neurochemistry International·Jesús Sánchez-NogueiroMaría Teresa Miras-Portugal
Dec 9, 2008·Experimental Eye Research·Jeremy D Rhodes, Julie Sanderson
Jul 8, 2009·Biophysical Journal·Patrick SchulzKlaus Fendler
Jun 11, 2016·Experimental Eye Research·Julie C LimPaul J Donaldson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Pflügers Archiv : European journal of physiology
Joel R GeverAnthony P D W Ford
Proceedings of the National Academy of Sciences of the United States of America
R R NeubigJ B Cohen
Proceedings of the National Academy of Sciences of the United States of America
G L Hazelbauer, J P Changeux
© 2022 Meta ULC. All rights reserved