Molecular Identification and Localization of Filamentous Symbiotic Bacteria Associated with the Hydrothermal Vent Annelid Alvinella pompejana.

Applied and Environmental Microbiology
S C CaryD Desbruyères

Abstract

Alvinella pompejana is a polychaetous annelid that inhabits high-temperature environments associated with active deep-sea hydrothermal vents along the East Pacific Rise. A unique and diverse epibiotic microflora with a prominent filamentous morphotype is found associated with the worm's dorsal integument. A previous study established the taxonomic positions of two epsilon proteobacterial phylotypes, 13B and 5A, which dominated a clone library of 16S rRNA genes amplified by PCR from the epibiotic microbial community of an A. pompejana specimen. In the present study deoxyoligonucleotide PCR primers specific for phylotypes 13B and 5A were used to demonstrate that these phylotypes are regular features of the bacterial community associated with A. pompejana. Assaying of other surfaces around colonies of A. pompejana revealed that phylotypes 13B and 5A are not restricted to A. pompejana. Phylotype 13B occurs on the exterior surfaces of other invertebrate genera and rock surfaces, and phylotype 5A occurs on a congener, Alvinella caudata. The 13B and 5A phylotypes were identified and localized on A. pompejana by in situ hybridization, demonstrating that these two phylotypes are, in fact, the prominent filamentous bacteria on the dorsal...Continue Reading

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Citations

Apr 4, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K C WarrenS C Cary
Apr 1, 2000·Applied and Environmental Microbiology·A R SipeS C Cary
Mar 28, 2002·Applied and Environmental Microbiology·Andreas TeskeHolger W Jannasch
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·M L MiroshnichenkoE Stackebrandt
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Apr 1, 2003·FEMS Microbiology Ecology·Julie A HuberJohn A Baross
May 26, 1999·Critical Reviews in Microbiology·D CorsaroM Valassina
Sep 6, 2003·Applied and Environmental Microbiology·Annette Summers EngelMichael Wagner
May 3, 2006·Nature Reviews. Microbiology·Barbara J CampbellKen Takai
May 7, 2004·Applied and Environmental Microbiology·Shana K GoffrediRobert C Vrijenhoek
Dec 18, 2007·Applied and Environmental Microbiology·Charles K LeeRoy M Daniel
Jun 4, 2011·Canadian Journal of Microbiology·C William YeungCharles W Greer
Oct 12, 2010·Journal of Nucleic Acids·Sayo KashiwagiShigenori Iwai
Feb 24, 2012·Canadian Journal of Microbiology·Ruyin LiuXinchun Liu
Jun 28, 2015·Applied and Environmental Microbiology·Nancy Weiland-BräuerRuth A Schmitz
Apr 12, 2001·Nature·G W LutherS C Cary
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Feb 12, 2004·Environmental Microbiology·Karine AlainMarie-Anne Cambon-Bonavita
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Sep 17, 2008·Nature Reviews. Microbiology·Nicole DubilierChristian Lott
Dec 14, 2005·Environmental Microbiology·Elizabeth A McClimentS Craig Cary

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