Molecular identification of Babesia parasites isolated from Ixodes ricinus ticks collected in northwestern Poland

The Journal of Parasitology
Norman J PieniazekBogumiła Skotarczak

Abstract

In the present study, PCR has been applied to detect and analyze DNA of Babesia spp. extracted from Ixodes ricinus ticks. Collection of I. ricinus was made in 6 forested areas of Zachodniopomorskie Voivodship, Poland, during 2 seasonal peaks of tick activity, i.e., spring and autumn, 2001. In total, 1,328 I. ricinus were collected and processed for PCR with F34 and R323 primers. Babesia spp. was detected in 28 (2% of 1,328 tested) ticks; 26 were identified as B. divergens. The other 2 were identified as B. microti. PCR was conducted with 18S rRNA specific primers and sequencing was processed to precisely identify and compare these isolates with B. microti and B. divergens sequences from Europe, North America, and Asia obtained from the GenBank. Analysis revealed that sequences of B. microti from northwestern Poland are almost identical (99.94%) with those referred to as "Munich strain"; both form a clade different from other European strains, as well as those from Asia and North America (called B. microti, sensu stricto). An investigation performed with B. divergens sequences showed that the sequence from northwestern Poland is 99.94% homologous to an isolate from Ireland ("Purnel"), and differs in just a few nucleotides from o...Continue Reading

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Citations

Jun 2, 2012·Vector Borne and Zoonotic Diseases·Angelina Wójcik-FatlaJacek Dutkiewicz
Mar 12, 2013·Vector Borne and Zoonotic Diseases·Renata Welc-FalęciakJerzy M Behnke
May 23, 2014·Vector Borne and Zoonotic Diseases·Eva R KallioPhillip C Watts
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Jan 6, 2009·Medical and Veterinary Entomology·J García-SanmartínA Hurtado
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Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·V A RarN V Tikunova
Aug 26, 2016·Vector Borne and Zoonotic Diseases·Marta ArsuagaEstrella Montero
May 2, 2014·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·G DoblerM Niedrig
Apr 19, 2012·The Journal of Parasitology·A MovilaA Alekhnovici

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Datasets Mentioned

BETA
AY789075
AY789076
AY648876

Methods Mentioned

BETA
PCR

Software Mentioned

DNAMAN

Related Concepts

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Babesiosis (ASM)

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

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