Molecular identification of Blastocystis sp. subtypes in water samples collected from Black sea, Turkey

Acta Tropica
Zeynep KolorenPanagiotis Karanis

Abstract

The aim of this study was to identify the subtypes of Blastocystis sp. and complete a phylogenetic analysis of 268 water samples that were collected from the Samsun, Amasya and Sinop Provinces of the Black Sea in Turkey, between the years 2011 and 2014. Blastocystis sp. was investigated in 48 uncultured sea water samples that were collected from 4 sites within the Sinop Province. A total of 100 river water samples were collected from 37 sites in the Samsun Province and 120 river water samples were collected from 10 sampling sites within the Amasya Province. The small subunit (SSU) rDNA gene Polymerase chain reaction (PCR) were performed for the detection of Blastocytis sp. and the PCR-positive samples were sequenced. Subsequently, the (SSU) rDNA sequences were aligned by Bioedit and phylogenetic trees were constructed for Blastocystis with reference to the genotypes from GenBank. Blastocystis sp. were found in 3 out of the 75 (4%) river water samples that were collected from the Samsun Province. Six of the 120 (5%) river water samples and 1 out of the 48 (2%) seawater samples were positive for Blastocystis in the Amasya and Sinop Provinces. There were two different subtypes (ST; 1 and 3) found from sequencing all of the samples...Continue Reading

Citations

Aug 14, 2020·International Journal of Environmental Health Research·Małgorzata Adamska
Nov 30, 2018·Parasitology Research·Jenny G MaloneyMonica Santin
Feb 11, 2019·Parasitology Research·Zeynep Erdem AynurSema Ertuğ
Oct 14, 2020·Research in Veterinary Science·Josephine S Y HublinMonica Santin

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