Molecular identification of four novel cytochrome P450 genes related to the development of resistance of Spodoptera exigua (Lepidoptera: Noctuidae) to chlorantraniliprole

Pest Management Science
Xuegui WangQunfang Yang

Abstract

The beet armyworm, Spodoptera exigua, is an omnivorous insect that damages a variety of crops worldwide. Chlorantraniliprole is a new diamide insecticide that acts on the ryanodine receptors in insects. The aim of this study was to explore key genes related to the development of chlorantraniliprole resistance in S. exigua. Transcriptomes were compared between beet armyworms from a susceptible laboratory strain (Sus-Lab) and Sus-Lab screened with LC25 sublethal doses of chlorantraniliprole for six generations (SE-Sel). Ten of 11 cytochrome P450 genes with upregulated expression verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in SE-Sel strains were detected in SE-PZ16 and SE-ZY16, two extremely resistant field populations. In addition, expression of four new cytochrome P450 genes, CYP9A21v1, CYP9A21v2, CYP9A21v3 and CYP9A21v4, was shown in the two field populations and was significantly higher in the SE-Sel strain than in the Sus-Lab strain (P < 0.05). Their full-length and protein tertiary structures were also cloned and predicted. The function of CYP9A21v3 was analysed by RNA interference, and the relative expression of CYP9A21v3 in the SE-ZY16 population after feeding on dsRNA was lower than ...Continue Reading

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Citations

May 10, 2019·International Journal of Molecular Sciences·Muhammad HafeezMo Wang
May 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Llewellyn GreenCharles Robin
Aug 21, 2020·Pest Management Science·Karina Lucas Silva-BrandãoJosé Roberto Trigo
Aug 24, 2020·Pesticide Biochemistry and Physiology·Li XuChengju Wang
May 21, 2021·Journal of Agricultural and Food Chemistry·Xiaochun ZengQingli Shang
Jul 25, 2021·Pesticide Biochemistry and Physiology·Ying ShaoZhong-Zheng Gui
Sep 2, 2021·Insect Biochemistry and Molecular Biology·Dries AmezianGaëlle Le Goff
Oct 29, 2021·Environmental Science and Pollution Research International·Muhammad HafeezYaobin Lu

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