Molecular Identification of Helicoverpa armigera (Lepidoptera: Noctuidae: Heliothinae) in Argentina and Development of a Novel PCR-RFLP Method for its Rapid Differentiation From H. zea and H. gelotopoeon

Journal of Economic Entomology
J D ArneodoA Sciocco-Cap

Abstract

Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae: Heliothinae) is among the most voracious global pests of agriculture. Adults of this species were identified recently in northern Argentina by dissection of male genitalia. In this work, a rapid and simple molecular tool was designed to distinguish H. armigera from the morphologically similar indigenous bollworms Helicoverpa zea (Boddie) and Helicoverpa gelotopoeon (Dyar), regardless of the life stage. Amplification of partial COI gene with a new primer pair, and subsequent digestion with endonuclease HinfI, yielded different RFLP profiles for the three main Helicoverpa pests currently present in South America. The method was validated in Helicoverpa specimens collected across Argentina, whose identity was further corroborated by COI sequencing and phylogenetic analysis. The data reported here constitute the first molecular confirmation of this pest in the country. The survey revealed the occurrence of H. armigera in northern and central Argentina, including the main soybean- and maize-producing area.

References

Jul 18, 2008·Bulletin of Entomological Research·G T BehereP Batterham
Jan 24, 2014·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·Thomas K Walsh

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Citations

Jul 21, 2016·Current Opinion in Insect Science·C A BlancoE Willink
Aug 17, 2021·Neotropical Entomology·Daniele Heloísa Pinheiro, Fernando Hercos Valicente

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