Molecular identification of human tuberculosis in recent and historic bone tissue samples: The role of molecular techniques for the study of historic tuberculosis

American Journal of Physical Anthropology
Albert R ZinkAndreas G Nerlich

Abstract

We describe the molecular identification of the M. tuberculosis complex DNA in bone tissue samples from recent and historic populations. In a first set, archival paraffin material from vertebral bodies of 12 recent cases with clinically/microbiologically proven tuberculosis was compared to 12 further cases without tuberculosis. While eight TB cases revealed a specific mycobacterial amplification product, none of the controls was positive. Interestingly, one case with tuberculous sepsis (Landouzy sepsis), five cases with tuberculous spread beyond the primarily affected organ (i.e., lymph node or miliar involvement), and also two of six cases with restricted pulmonary tuberculosis reacted positively in the vertebral specimens. This indicates that a molecular analysis can detect mycobacteria even in unremarkable bone tissue, proving that organ tuberculosis is present. In addition, the extent of spread is of high significance for the frequency of positive reactions. In addition, we investigated a series of vertebral samples coming from an Egyptian population of the necropolis of Thebes-West dating to approximately 1450-500 BC. In this group of 36 cases, three of five cases with typical macromorphological signs for tuberculous spond...Continue Reading

References

Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Feb 1, 1991·Genomics·Y NakahoriY Nakagome
May 1, 1990·The Journal of Infectious Diseases·K D EisenachJ T Crawford
Dec 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P G RobeyM F Young
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·W L SaloT A Holcomb
Jun 1, 1994·American Journal of Clinical Pathology·R A GhosseinA R Rabson
Jul 1, 1993·American Journal of Physical Anthropology·A G NerlichK von der Mark
Nov 1, 1996·Journal of Clinical Microbiology·T J HellyerK D Eisenach
Nov 20, 1997·Lancet·A G NerlichH G Hagedorn
Feb 14, 1998·Nature Genetics·F R SantosC Tyler-Smith
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·M DrancourtD Raoult
Nov 27, 1998·Letters in Applied Microbiology·H D DonoghueD E Minnikin
Jan 8, 1999·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·E CrubézyD Montagnon
May 6, 1999·American Journal of Physical Anthropology·F GuhlA C Aufderheide
Jan 5, 2000·American Journal of Physical Anthropology·C J Kolman, N Tuross
Jun 13, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·J Y LiC S Ng
Sep 16, 2000·American Journal of Clinical Pathology·C J HaasA G Nerlich
Oct 24, 2000·American Journal of Physical Anthropology·C J HaasA G Nerlich
Nov 18, 2000·Archives of Pathology & Laboratory Medicine·A ZinkA G Nerlich
Apr 6, 2001·Journal of Medical Microbiology·A ZinkA G Nerlich
Jan 9, 2003·Journal of Clinical Microbiology·Albert R ZinkAndreas G Nerlich

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Citations

Nov 1, 2011·International Orthopaedics·Anil Kumar JainIsh Kumar Dhammi
Jun 20, 2012·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Carlos Pigrau-Serrallach, Dolores Rodríguez-Pardo
Jul 20, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Philip A MackowiakJane E Buikstra
Apr 13, 2006·Proceedings. Biological Sciences·Helen D Donoghue, Mark Spigelman
Nov 19, 2011·Homo : internationale Zeitschrift für die vergleichende Forschung am Menschen·K L HollowayM Henneberg
Nov 22, 2005·Infectious Disease Clinics of North America·Michael Gardam, Sue Lim
Aug 19, 2009·Journal of Forensic Sciences·Kenneth D Eilert, David R Foran
Nov 6, 2009·American Journal of Physical Anthropology·Anne C StoneCharlotte A Roberts
Jul 27, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H W WitasJ J Gładykowska-Rzeczycka
Jul 15, 2015·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Sandra AssisFrancisca Alves Cardoso
Feb 11, 2015·Global Heart·Albert ZinkJohannes Krause
Apr 11, 2009·Interdisciplinary Perspectives on Infectious Diseases·Andreas G Nerlich, Sandra Lösch
Mar 7, 2007·Journal of the Royal Society of Medicine·Heather Milburn
Aug 3, 2016·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·H H Hans de Boer, A E Lida van der Merwe
Jul 3, 2013·Virchows Archiv : an International Journal of Pathology·Kais HusseinAndreas G Nerlich
Jul 3, 2010·The Journal of Bone and Joint Surgery. British Volume·A K Jain
Jan 22, 2019·International Journal of Paleopathology·Dorthe Dangvard PedersenJesper Lier Boldsen
Oct 16, 2020·Human Molecular Genetics·Yehia Z GadSomaia Ismail
Nov 27, 2018·Chemical Reviews·Alex ChaoCelia W Goulding

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