Abstract
Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.
References
Oct 29, 2005·Journal of Clinical Pathology·R BialekV Rickerts
Mar 17, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Volker RickertsRalf Bialek
Apr 25, 2007·Antimicrobial Agents and Chemotherapy·Nikolaos G AlmyroudisShimon Kusne
Aug 22, 2007·Medical Mycology·S Arunmozhi BalajeeMary E Brandt
May 16, 2008·Journal of Clinical Microbiology·D Jane HataNancy L Wengenack
Jul 10, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Georgios ChamilosDimitrios P Kontoyiannis
Dec 17, 2008·Journal of Clinical Microbiology·S A BalajeeB L Wickes
Sep 18, 2009·Journal of Clinical Microbiology·Dea Garcia-HermosoEric Dannaoui
Oct 19, 2010·Medical Mycology·Odile CabaretStéphane Bretagne
Apr 22, 2011·Journal of Clinical Microbiology·Sarah P HammondFrancisco M Marty
Feb 2, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·F LanternierUNKNOWN French Mycosis Study Group
Oct 27, 2012·Journal of Clinical Microbiology·Jeong Hwan ShinYi-Wei Tang
Feb 20, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Laurence MillonFrederic Grenouillet
Mar 8, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M J BuitragoM Cuenca-Estrella
Mar 22, 2013·Journal of Clinical Microbiology·Patricia J SimnerNancy L Wengenack
Jun 25, 2014·Emerging Infectious Diseases·Dounia BitarFrançoise Dromer
Citations
Dec 27, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L MillonUNKNOWN French Mycosis Study Group
Jun 11, 2016·Jundishapur Journal of Microbiology·Ardeshir ZiaeeJamal Hashemi
Aug 4, 2016·Expert Review of Molecular Diagnostics·Matthew William McCarthy, Thomas J Walsh
Sep 25, 2016·Medical Mycology·Alexandre Alanio, Stéphane Bretagne
Oct 30, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthieu LegrandAlexandre Alanio
Dec 3, 2016·Journal of Medical Microbiology·Jan SpringerJuergen Loeffler
Mar 15, 2018·Medical Mycology·Andoni Ramirez-GarciaFernando L Hernando
Sep 12, 2018·Expert Review of Molecular Diagnostics·Sanjeet S Dadwal, Dimitrios P Kontoyiannis
Aug 3, 2018·Journal of Clinical Microbiology·Clara BaldinAshraf S Ibrahim
Nov 20, 2019·Journal of Fungi·Grit WaltherOliver Kurzai
Mar 17, 2017·F1000Research·Alexandre Alanio, Stéphane Bretagne
Mar 30, 2017·The Journal of Antimicrobial Chemotherapy·Matteo Bassetti, Emilio Bouza
Oct 3, 2018·F1000Research·Benoit PilmisFanny Lanternier
Jun 4, 2020·MBio·Abdullah AlqarihiAshraf S Ibrahim
Mar 23, 2019·Journal of Fungi·Laurence MillonAnne-Pauline Bellanger
Jul 7, 2019·Journal of Fungi·Thomas J WalshDimitrios P Kontoyiannis
Nov 6, 2020·Journal of Fungi·Anna SkiadaMaria Drogari-Apiranthitou
Feb 13, 2021·Journal of Fungi·Zoe Freeman WeissSophia Koo
Apr 4, 2021·Journal of Fungi·Guillermo Garcia-Effron
Dec 23, 2021·Internal Medicine Journal·Olivia Bupha-IntrUNKNOWN Australasian Antifungal Guidelines Steering Committee