Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
A AlanioFrench Mycosis Study Group

Abstract

Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.

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Citations

Dec 27, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L MillonUNKNOWN French Mycosis Study Group
Jun 11, 2016·Jundishapur Journal of Microbiology·Ardeshir ZiaeeJamal Hashemi
Aug 4, 2016·Expert Review of Molecular Diagnostics·Matthew William McCarthy, Thomas J Walsh
Sep 25, 2016·Medical Mycology·Alexandre Alanio, Stéphane Bretagne
Oct 30, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthieu LegrandAlexandre Alanio
Dec 3, 2016·Journal of Medical Microbiology·Jan SpringerJuergen Loeffler
Mar 15, 2018·Medical Mycology·Andoni Ramirez-GarciaFernando L Hernando
Sep 12, 2018·Expert Review of Molecular Diagnostics·Sanjeet S Dadwal, Dimitrios P Kontoyiannis
Aug 3, 2018·Journal of Clinical Microbiology·Clara BaldinAshraf S Ibrahim
Nov 20, 2019·Journal of Fungi·Grit WaltherOliver Kurzai
Mar 17, 2017·F1000Research·Alexandre Alanio, Stéphane Bretagne
Mar 30, 2017·The Journal of Antimicrobial Chemotherapy·Matteo Bassetti, Emilio Bouza
Oct 3, 2018·F1000Research·Benoit PilmisFanny Lanternier
Mar 23, 2019·Journal of Fungi·Laurence MillonAnne-Pauline Bellanger
Nov 6, 2020·Journal of Fungi·Anna SkiadaMaria Drogari-Apiranthitou
Dec 23, 2021·Internal Medicine Journal·Olivia Bupha-IntrUNKNOWN Australasian Antifungal Guidelines Steering Committee

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