Molecular identification of mutations associated with anti-tuberculosis drug resistance among strains of Mycobacterium tuberculosis

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
Said H AbbadiRobert C Cooksey

Abstract

Understanding the etiologic organism, antimicrobial resistance mechanisms, and transmission of multidrug-resistant tuberculosis (MDR-TB) can be of great value in optimizing strategies to control and prevent its development and transmission. One hundred and fifty-five Mycobacterium tuberculosis complex isolates from patients with pulmonary tuberculosis (TB) in Cairo, Egypt were studied. In vitro drug susceptibility testing against rifampin (RIF), isoniazid (INH), streptomycin (SM), ethambutol (EMB), and pyrazinamide (PZA) was performed. Resistance was studied by the standard agar proportion method. Single strand conformation polymorphism (SSCP) and DNA sequence analysis were used to detect mutations in the genes that encode resistance to rpoB, katG, rpsL, and embB. Among 155 consecutive M. tuberculosis isolates, 25 (16.1%) were MDR-TB; 13 of these were from newly diagnosed untreated cases, 12 were from re-treated cases, and none of the MDR-TB isolates had matching IS6110 fingerprints. Among the MDR-TB isolates, rpoB mutations were found in 76% of RIF-resistant isolates, katG mutations were found in 47.1% of INH-resistant isolates, rpsL mutations were found in 55.6% of SM-resistant isolates, and embB mutations were found in 36.4%...Continue Reading

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Citations

Jul 19, 2012·Journal of Biomolecular Structure & Dynamics·Jyothi PadiadpuNagasuma Chandra
Sep 2, 2011·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Marcelo MiyataClarice Queico Fujimura Leite
Oct 17, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A ShiblZ Memish
Oct 21, 2016·ACS Infectious Diseases·Jyothi PadiadpuNagasuma Chandra
Sep 4, 2014·Chemical Communications : Chem Comm·Danishmalik Rafiq SayyedTaisun Kim
Sep 4, 2018·Expert Review of Anti-infective Therapy·Suhail AhmadNoura M Al-Mutairi
Mar 25, 2021·Infection and Drug Resistance·Liang DuLing Zhou

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