Molecular identification of Mycobacterium avium subsp. silvaticum by duplex high-resolution melt analysis and subspecies-specific real-time PCR

Journal of Clinical Microbiology
Zsuzsanna RónaiÁdám Dán

Abstract

Accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epidemiological studies. The subspecies of Mycobacterium avium have different attributes but coincide in their zoonotic potential. Our knowledge about M. avium subsp. silvaticum is limited, since its identification is uncertain. Mycobacterium avium subsp. avium and M. avium subsp. silvaticum can be discriminated from each other based only on phenotypic characteristics, as they have almost identical genome sequences. Here we describe the development of a diagnostic method which enables the molecular identification of M. avium subsp. silvaticum and discrimination from M. avium subsp. avium based on genomic differences in a duplex high-resolution melt and M. avium subsp. silvaticum-specific mismatch real-time PCR. The developed assay was tested on reference strains and 199 field isolates, which were analyzed by phenotypic methods previously. This assay not only identified all 63 M. avium subsp. silvaticum and 138 M. avium subsp. avium strains correctly but also enabled the detection of mixed M. avium subsp. avium-M. avium subsp. silvaticum cultures. This is the first time that such a large panel of strains has...Continue Reading

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Citations

Nov 4, 2015·Annual Review of Animal Biosciences·Lingling LiVivek Kapur
Mar 12, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Zsuzsanna RónaiMiklós Gyuranecz
Feb 2, 2018·Clinical Microbiology Reviews·Betty A ForbesMichael L Wilson
May 5, 2018·BMC Infectious Diseases·Roland DielWouter Hoefsloot
Jan 28, 2021·Animals : an Open Access Journal From MDPI·Vasilios TsiourisIoanna Georgopoulou
Apr 4, 2021·International Journal of Molecular Sciences·Min-Kyoung Shin, Sung Jae Shin

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