Molecular identification of mycorrhizal fungi in Neuwiedia veratrifolia (Orchidaceae)

Molecular Phylogenetics and Evolution
K A KristiansenH N Rasmussen

Abstract

We here apply a previously described method for identification of single peloton orchid mycorrhiza to a key orchid group and extend the usefulness in the heterobasidiomycetes of an existing fungal database for identification of mycorrhizal fungi. We amplified and sequenced mitochondrial ribosomal large subunit DNA from fungi in roots of Neuwiedia veratrifolia (Orchidaceae), a member of the small subfamily Apostasioideae that is sister to the remainder of Orchidaceae, and used the extended database to identify the mycorrhizal fungi. Sequences from fungi cultured from Neuwiedia roots and from direct peloton amplifications were analyzed cladistically with sequences determined from reference fungal collections and published sequences. The fungi from Neuwiedia are referred to the heterobasidiomycetous orders Tulasnellales and Ceratobasidiales, indicating that apostasioids utilize the same fungi as other photosynthetic orchids. The majority of Neuwiedia mycobionts came together in a clade with Tulasnella species, but some were most closely related to Thanatephorus. In some cases members of these two clades were isolated from the same orchid plant, providing another example of multiple mycobionts occurring in a single plant.

References

Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·D L Taylor, T D Bruns
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·D S HibbettM J Donoghue
Feb 14, 2002·Molecular Phylogenetics and Evolution·Manfred Binder, David S Hibbett
Jul 9, 2002·Molecular Phylogenetics and Evolution·Jean-Marc MoncalvoOrson K Miller
Feb 1, 1999·American Journal of Botany·K M CameronD H Goldman
Feb 1, 1999·American Journal of Botany·J V Freudenstein, F N Rasmussen

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Citations

Jun 22, 2007·Mycorrhiza·John D W Dearnaley
Nov 4, 2006·Mycological Research·Juan Pablo SuárezIngrid Kottke
Jun 5, 2007·Evolution; International Journal of Organic Evolution·Richard P SheffersonYung-I Lee
Aug 15, 2015·Annals of Botany·Hanne N RasmussenTamara Těšitelová

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