Molecular identification of rickettsial endosymbionts in the non-phagotrophic volvocalean green algae.

PloS One
Kaoru KawafuneH Nozaki

Abstract

The order Rickettsiales comprises gram-negative obligate intracellular bacteria (also called rickettsias) that are mainly associated with arthropod hosts. This group is medically important because it contains human-pathogenic species that cause dangerous diseases. Until now, there has been no report of non-phagotrophic photosynthetic eukaryotes, such as green plants, harboring rickettsias. We examined the bacterial endosymbionts of two freshwater volvocalean green algae: unicellular Carteria cerasiformis and colonial Pleodorina japonica. Epifluorescence microscopy using 4'-6-deamidino-2-phenylindole staining revealed the presence of endosymbionts in all C. cerasiformis NIES-425 cells, and demonstrated a positive correlation between host cell size and the number of endosymbionts. Strains both containing and lacking endosymbionts of C. cerasiformis (NIES-425 and NIES-424) showed a >10-fold increase in cell number and typical sigmoid growth curves over 192 h. A phylogenetic analysis of 16 S ribosomal (r)RNA gene sequences from the endosymbionts of C. cerasiformis and P. japonica demonstrated that they formed a robust clade (hydra group) with endosymbionts of various non-arthropod hosts within the family Rickettsiaceae. There were ...Continue Reading

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Citations

Dec 15, 2015·Biotechnology Advances·Rishiram RamananHee-Sik Kim
Apr 25, 2015·Environmental Microbiology·Frederik SchulzMatthias Horn
Feb 12, 2015·PloS One·Kaoru KawafuneHisayoshi Nozaki
Sep 23, 2016·Frontiers in Microbiology·Josselin LupetteSophie Sanchez-Ferandin
Aug 21, 2019·Pathogens and Global Health·Thilini Dilhara Jayasena KaluarachchiRajitha Wickremasinghe
Feb 25, 2017·Frontiers in Microbiology·Kassandra E ZailaWei-Jen Chang

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Datasets Mentioned

BETA
AB688628
AB688629
AB688625
AB688626
AB688624
AB688627

Methods Mentioned

BETA
transmission electron microscopy
PCR

Software Mentioned

probeCheck
ARB
BLASTn
DINAMelt
PhyML
PAUP
CLUSTAL X
MODELTEST

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