Molecular identification of T cell-specific antigens on human T lymphocytes and thymocytes

European Journal of Immunology
L C AnderssonH Rodt

Abstract

We have identified membrane glycoproteins which carry T cell-specific antigens on human T lymphocytes and thymocytes. Purified cells were surface-labeled with NaB3H4 after treatment with neuraminidase and galactose oxidase. Immunoprecipitations were performed with rabbit anti-human T cell-specific antibodies using coprecipitation with protein A-containing staphylococci strain Cowan I. The labeled membrane glycoproteins and the precipitates were subjected to polyacrylamide slab gel electrophoresis and visualized by fluorography. The antibodies specifically precipitated 4 proteins called GP 200, GP 180, GP 165 and GP 160 (mol. wts. = 200,000, 180,000, 165,000 and 160,000) from surface-labeled T lymphocytes and low-density (medullary) thymocytes. The GP 200 and GP 180 were not labeled on high-density (cortical) thymocytes. A protein with a mol. wt. of 45,000 was precipitated from thymocytes. Another glycoprotein on T lymphocytes and thymocytes with a mol. wt. similar to that of mouse and rat Thy-1 or theta antigen (mol. wt. 24,000) reacted with the antibodies.

References

Dec 1, 1975·The Biochemical Journal·M Letarte-MuirheadA F Williams
Mar 1, 1979·The Journal of Experimental Medicine·R Dalchau, J W Fabre
Oct 15, 1979·Molecular and Cellular Biochemistry·L C Andersson, C G Gahmberg
Mar 1, 1979·European Journal of Immunology·A J McMichaelC Milstein
Aug 1, 1978·The Journal of Experimental Medicine·C G Gahmberg, L C Andersson
Jun 1, 1976·Scandinavian Journal of Immunology·P Häyry, L C Andersson
Jan 1, 1972·Scandinavian Journal of Immunology·S S Fröland
Feb 1, 1973·The Journal of Clinical Investigation·R C WilliamsF D Lindström
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Sep 1, 1964·The Journal of Experimental Medicine·A E REIF, J M ALLEN

❮ Previous
Next ❯

Citations

Oct 1, 1994·Veterinary Immunology and Immunopathology·F A ZuckermannJ K Lunney
Oct 1, 1994·Veterinary Immunology and Immunopathology·F A ZuckermannR M Binns
Oct 1, 1988·Immunology Today·M L Thomas, L Lefrançois
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W JuddJ L Strominger
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·O H IrigoyenL Chess
Jan 1, 1985·Critical Reviews in Oncology/hematology·E Thiel
Sep 16, 2014·Acta Pharmacologica Sinica·Rong-Jun HeZhong-Yin Zhang
Sep 15, 1983·International Journal of Cancer. Journal International Du Cancer·A M Lebacq-VerheydenM F Greaves
Mar 1, 1990·European Journal of Immunology·L K GoffA G Fisher
Jun 1, 1987·Immunological Investigations·S A Gregory, W L Myers
Oct 1, 1981·Cellular Immunology·L C AnderssonC G Gahmberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.