Molecular identification of zoonotic hookworms in dogs from four counties of Kenya

Journal of Helminthology
Erastus MulingeThomas Romig

Abstract

All canine hookworms are known to be zoonotic, causing infections ranging from transient skin irritations to prolonged 'creeping eruptions', eosinophilic enteritis and even patent intestinal infections. There is little information on canine hookworm species and their public health significance in sub-Saharan Africa. This study determined the prevalence and species of hookworms in dogs from different climatic zones of Kenya. Dog faecal samples were collected from the environment, and hookworm eggs were isolated by zinc chloride flotation and subjected to DNA extraction. Polymerase chain reaction (PCR) assays targeting the internal transcribed spacer (ITS) 1 and 2, 5.8S and 28S ribosomal RNA of Ancylostoma spp. and Uncinaria stenocephala were performed, and hookworm species were identified by PCR-restriction fragment length polymorphism (RFLP) or DNA sequencing. Hookworm eggs were detected by microscopy in 490/1621 (30.23%, 95% CI 28.01-32.54) faecal samples. Estimates of faecal prevalence were high in counties receiving higher rainfall (Narok 46.80%, Meru 44.88%) and low in those with a more arid climate (Isiolo 19.73%, Turkana 11.83%). In a subset of 70 faecal samples, Ancylostoma caninum (n = 59) was the most common species, f...Continue Reading

References

Jan 1, 1968·Transactions of the Royal Society of Tropical Medicine and Hygiene·T A Miller
Dec 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T JelinekT Löscher
May 25, 1993·Nucleic Acids Research·R B GasserI Beveridge
Jun 1, 1996·Parasite : Journal De La Société Française De Parasitologie·R MalgorI Yarzábal
Feb 27, 1998·Veterinary Parasitology·D S LindsayB L Blagburn
May 18, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E Caumes
Sep 15, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·O BouchaudJ P Coulaud
Oct 3, 2000·Advances in Parasitology·D W Crompton
Sep 5, 2001·The British Journal of Dermatology·V Blackwell, F Vega-Lopez
Aug 14, 2002·The British Journal of Dermatology·P Del GiudiceP Dellamonica
Sep 10, 2002·Dermatology : International Journal for Clinical and Investigative Dermatology·Natalie A SchaubStanislaw A Buechner
Dec 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·B GalantiS Nardiello
Dec 14, 2002·The American Journal of Tropical Medicine and Hygiene·Rebecca J TraubR C Andrew Thompson
Dec 6, 2003·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Jacob Karl FrenkelMike Dobesh
Aug 25, 2004·Veterinary Parasitology·Rebecca J TraubR C Andrew Thompson
May 2, 2006·Advances in Parasitology·S BrookerD A P Bundy
May 23, 2006·Veterinary Parasitology·Leandra M Clara e SilvaElida M L Rabelo
Sep 26, 2006·BMC Veterinary Research·Laura RinaldiGiuseppe Cringoli
Aug 23, 2007·Emergency Medicine Journal : EMJ·Ravichandra Kelkar
Aug 31, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Jessica Sanchez WerneckSoraya Sgambatti Andrade
Sep 22, 2007·Journal of Travel Medicine·Patrick Hochedez, Eric Caumes
Jun 10, 2008·Indian Journal of Pediatrics·Sunil SaharanVinod K Paul
Mar 12, 2009·International Journal for Parasitology·Marion HüttnerThomas Romig
Mar 20, 2009·The Onderstepoort Journal of Veterinary Research·B DavoustG Bourdoiseau
Mar 2, 2010·Trends in Parasitology·Dwight D BowmanKevin R Kazacos
Jul 9, 2010·Journal of Medical Case Reports·Luna DhirMark T Watts
Aug 3, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Megumi SatoJitra Waikagul
Apr 5, 2011·The American Journal of Tropical Medicine and Hygiene·Vittaya JiraanankulSaovanee Leelayoova
Sep 15, 2011·Revista Do Instituto De Medicina Tropical De São Paulo·Willian Marinho Dourado CoelhoKatia Denise Saraiva Bresciani
Apr 12, 2012·The American Journal of Tropical Medicine and Hygiene·James V ConlanR C Andrew Thompson
Apr 28, 2012·Parasites & Vectors·Rachel L Pullan, Simon J Brooker
May 9, 2012·Parasites & Vectors·Mohammed A K MahdyJohari Surin
Oct 4, 2012·Geospatial Health·Ntombi B MudendaPenelope Vounatsou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.