Molecular identification using ITS sequences and genome shuffling to improve 2-deoxyglucose tolerance and xylanase activity of marine-derived fungus, Aspergillus sp. NRCF5

Applied Biochemistry and Biotechnology
Ahmed M A El-Bondkly

Abstract

During the screening of xylanolytic enzyme from marine-derived fungi isolated from the inner tissue of Egyptian soft coral Rhytisma sp., one strain, NRCF5, exhibited high enzyme activity with 0.1 % (w/v) antimetabolite 2-deoxyglucose (2DG) tolerance. This fungal strain was identified as Aspergillus sp. NRCF5 based on its morphological characteristics and internal transcribed spacer (ITS) sequences. The ITS region of hyperactive xylanolytic strain (NRCF5) was amplified, sequenced, and submitted to GenBank (accession no. JQ277356). To apply the fundamental principles of genome shuffling in breeding of xylanase-producing fungi, marine-derived fungus Aspergillus sp. NRCF5 was used as starting strain in this work and applied for induction of genetic variability using different combinations and doses of mutagens. Five mutants with high xylanase activity and 0.25 % (w/v) antimetabolite 2DG tolerance were obtained from the populations generated by the mutation of combination between ultraviolet irradiation (UV, 5 min) and N-methyl-N-nitro-N-nitrosoguanidine (NTG, 100 μg/ml) for 30 (UNA) and 60 (UNB)min as well as NTG (100 μg/ml) and ethidium bromide (250 μg/ml) for 30 (NEA) and 60 (NEB)min. Then, they were subjected for recursive proto...Continue Reading

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Citations

May 24, 2013·Applied Biochemistry and Biotechnology·Chao WangXinle Liang
Sep 18, 2013·Applied Biochemistry and Biotechnology·Wei LiZhiqun Liang
Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
Mar 25, 2017·PloS One·Ramón Alberto Batista-GarcíaJorge Luis Folch-Mallol
Jan 13, 2018·Applied Biochemistry and Biotechnology·Mervat Morsy Abbas Ahmed El-GendyAhmed Mohamed Ahmed El-Bondkly
Feb 17, 2017·Applied Biochemistry and Biotechnology·Mervat Morsy Abbas Ahmed El-GendyAhmed Mohamed Ahmed El-Bondkly
Sep 25, 2020·World Journal of Microbiology & Biotechnology·Liang ChenKe Bian
Jan 4, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Mohamed F AwadEsraa Ahmed Mohamed El-Bondkly
Apr 30, 2021·Saudi Journal of Biological Sciences·Mervat Morsy Abbas Ahmed El-GendyAhmed Mohamed Ahmed El-Bondkly

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