Molecular Imaging of the Glomerulus via Mesangial Cell Uptake of Radiolabeled Tilmanocept.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Zhengtao QinDavid R Vera

Abstract

An unmet need for the clinical management of chronic kidney disease is a predictive tool of kidney function during the first decade of the disease, when there is silent loss of glomerular function. The objective of this study was to demonstrate receptor-mediated binding of tilmanocept to CD206 within the kidney and provide evidence of kinetic sensitivity of this binding to renal function. Methods: Rats were positioned in a PET scanner with the liver and kidneys within the field of view. After an intravenous injection of 68Ga-IRDye800-tilmanocept, using 1 of 2 scaled molar doses (0.02 nmol/g, n = 5; or 0.10 nmol/g, n = 5), or coinjection (n = 3) of 68Ga-IRDye800-tilmanocept (0.10 nmol/g) and unlabeled tilmanocept (5.0 nmol/g), or a negative control, 68Ga-IRDye800-DTPA-galactosyl-dextran (0.02 nmol/g, n = 5), each animal was imaged for 20 min followed by a whole-body scan. Frozen kidney sections were stained for podocytes and CD206 using immunofluorescence. Molecular imaging of diabetic db/db mice (4.9 wk, n = 6; 7.3 wk, n = 4; 13.3 wk, n = 6) and nondiabetic db/m mice (n = 6) was performed with fluorescence-labeled 99mTc-tilmanocept (18.5 MBq, 2.6 nmol). Thirty minutes after injection, blood, liver, kidneys, and urine were assay...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y S Kanwar, M G Farquhar
Feb 1, 1992·The Anatomical Record·J R Nyengaard, T F Bendtsen
Oct 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D Schlondorff
Sep 2, 1966·Science·K P HummelD L Coleman
Oct 1, 1984·The Journal of Clinical Investigation·S M MauerF C Goetz
Jul 9, 1998·The New England Journal of Medicine·P FiorettoM Mauer
Dec 6, 2001·Metabolism: Clinical and Experimental·M P CohenC W Shearman
Mar 8, 2002·Investigative Radiology·William C EckelmanMartin Brechbiel
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Jenny SörenssonBörje Haraldsson
May 9, 2003·American Journal of Physiology. Renal Physiology·Kumar SharmaStephen R Dunn
Dec 15, 2004·Academic Radiology·Claude B SirlinRobert F Mattrey
Oct 26, 2005·Nuclear Medicine and Biology·David R VeraRobert F Mattrey
Dec 23, 2006·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Pantelis A SarafidisGeorge L Bakris
Mar 10, 2007·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Willemijn A K M WindtRichard P E van Dokkum
Jan 1, 2014·World Journal of Diabetes·Maria L Gonzalez SuarezAlessia Fornoni
Nov 7, 2014·World Journal of Nephrology·Soumeya Brachemi, Guillaume Bollée
Dec 17, 2014·World Journal of Diabetes·Gemma CurrieChristian Delles
May 23, 2015·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·An S De Vriese, Fernando C Fervenza
Sep 1, 2015·Nuclear Medicine and Biology·Zhengtao QinDavid R Vera
Jan 1, 2015·Nature Reviews. Disease Primers·Merlin C ThomasMark E Cooper
Oct 26, 2016·JCI Insight·Rintaro SaitoKumar Sharma
Apr 25, 2017·Diabetes Research and Clinical Practice·K OgurtsovaL E Makaroff
May 20, 2017·Clinical Journal of the American Society of Nephrology : CJASN·Radica Z AlicicKatherine R Tuttle
Jan 26, 2018·Nuclear Medicine and Biology·Malgorzata LipowskaAndrew T Taylor

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Citations

May 31, 2019·European Journal of Nuclear Medicine and Molecular Imaging·Rudolf A WernerTakahiro Higuchi
Sep 1, 2019·Annals of Nuclear Medicine·Jun ToyoharaTetsuro Tago
Feb 1, 2021·Medical Image Analysis·Israa AlnazerChristine Fernandez-Maloigne
Jul 1, 2021·Nature Reviews. Nephrology·Barbara M KlinkhammerPeter Boor

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