Molecular, immunological and neurophysiological evaluations for early diagnosis of neural impairment in seropositive leprosy household contacts

PLoS Neglected Tropical Diseases
Diogo Fernandes Dos SantosIsabela Maria Bernardes Goulart

Abstract

Household contacts constitute the highest risk group for leprosy development, and despite significant progress in the disease control, early diagnosis remains the primary goals for leprosy management programs. We have recruited 175 seropositive and 35 seronegative household contacts from 2014 to 2016, who were subjected to an extensive protocol that included clinical, molecular (peripheral blood qPCR, slit-skin smear qPCR, skin biopsy qPCR) and electroneuromyographic evaluations. The positivity of peripheral blood qPCR of seropositive contacts was 40.6% (71/175) whereas only 8.6% (3/35) were qPCR positive in seronegative contacts (p = 0.0003). For the slit-skin smear, only 4% (7/175) of seropositive contacts presented positive bacilloscopy, whereas the qPCR detected 47.4% (83/175) positivity in this group compared with only 17.1% (6/35) in seronegative contacts (p = 0.0009). In the ENMG evaluation of contacts, 31.4% (55/175) of seropositives presented some neural impairment, and 13.3% (4/35) in seronegatives (p = 0.0163). The presence of neural thickening conferred a 2.94-fold higher chance of ENMG abnormality (p = 0.0031). Seropositive contacts presented a 4.04-fold higher chance of neural impairment (p = 0.0206). The peripher...Continue Reading

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Citations

Mar 15, 2019·PloS One·Marlice Fernandes de OliveiraIsabela Maria Bernardes Goulart
Oct 31, 2019·Arquivos de neuro-psiquiatria·Diogo Fernandes Dos SantosIsabela Maria Bernardes Goulart
Feb 12, 2020·Neurology. Clinical Practice·Diogo Fernandes Dos SantosIsabela Maria Bernardes Goulart
Jul 28, 2020·Transactions of the Royal Society of Tropical Medicine and Hygiene·Diogo Fernandes Dos SantosIsabela Maria Bernardes Goulart
Jul 16, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Aline do Carmo GonçalvesMariane Martins de Araújo Stefani

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Methods Mentioned

BETA
biopsy
enzyme-linked immunosorbent assay
biopsies
PCR
ELISA

Software Mentioned

GraphPad Prism

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