PMID: 8974449Sep 1, 1995Paper

Molecular imprinting approach for the recognition of adenine in aqueous medium and hydrolysis of adenosine 5'-triphosphate

Bioconjugate Chemistry
J Mathew, O Buchardt

Abstract

Polymers capable of recognizing adenine in aqueous medium were developed by the molecular imprinting technique using methacrylic acid and 4(5)-vinylimidazole as comonomers with ethylene glycol dimethacrylate as the cross-linking agent under different polymerization conditions. The affinity of these polymers for adenine and other nucleotide bases was compared. The association constant for the binding of adenine to the polymer is calculated to be 4.3 x 10(3)M(-1). Furthermore, binding of adenosine 5'-triphosphate (ATP) to the polymers was evaluated, and an enhanced binding compared to adenine was observed. The binding of ATP is pH dependent, with the maximum around pH 3. Results have been explained based on the hydrogen bonding and ionic interactions between ATP and the ligands on the polymer matrix. The catalytic effect of these polymers for the hydrolysis of ATP is briefly discussed.

References

Oct 12, 1977·Journal of the American Chemical Society·G Lancelot
Feb 18, 1993·Nature·G VlatakisK Mosbach

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Citations

Oct 14, 2004·Analytical and Bioanalytical Chemistry·Vivek Babu Kandimalla, Hunagxian Ju
Jan 30, 2002·Journal of Pharmaceutical and Biomedical Analysis·Stephen E MoringJohn F Stobaugh
Dec 2, 2006·Biotechnology Progress·Nicholas W TurnerDavid W Britt
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·David ReigadaCarles Solsona
Sep 8, 2004·Advanced Drug Delivery Reviews·J Zachary Hilt, Mark E Byrne
Jan 6, 2006·Journal of Molecular Recognition : JMR·Cameron AlexanderMichael J Whitcombe
May 23, 2001·Journal of Agricultural and Food Chemistry·O RamströmO Brüggemann

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