Molecular insights into cold active polygalacturonase enzyme for its potential application in food processing

Journal of Food Science and Technology
L N Ramya, K K Pulicherla

Abstract

Pectin is a complex structural heteropolysaccharide that require numerous pectinolytic enzymes for its complete degradation. Polygalacturonase from mesophilic or thermophilic origin are being widely used in fruit and vegetable processing in the recent decades to degrade pectic substances. Recently cold active pectinases are finding added advantages over meso and thermophilic counterparts, to use in industrial scale particularly in food processing industry. They facilitate in conservation of several properties of foods so that the end product retains its naturality and also generates economic benefits. In the present study, Pseudoalteromonas haloplanktis, a well reported marine psychrophile is taken as a model organism for cold active polygalacturonase and is evaluated in comparision to the routinely used mesophilic and thermophilic enzymes by insicio approach. Polygalacturonase sequences from industrially important microbial sources were subjected to MEME and Pfam wherein motifs and domains involved in the conservation were analyzed. Dendrogram revealed sequence level similarity and motifs showed uniform distribution of conserved regions that are involved in important functions. It was also observed through clustalW analysis th...Continue Reading

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Citations

May 19, 2020·Journal of the Science of Food and Agriculture·Jing DaiJianwei Mao
Sep 25, 2019·Marine Drugs·Stefano BrunoCinzia Verde
Apr 28, 2019·Applied Microbiology and Biotechnology·Yumeng TangGuimin Zhang

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