Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System.

The Journal of Biological Chemistry
Serry KohMyung Hee Kim

Abstract

TodS is a sensor kinase that responds to various monoaromatic compounds, which either cause an agonistic or antagonistic effect on phosphorylation of its cognate response regulator TodT, and controls tod operon expression in Pseudomonas putida strains. We describe a molecular sensing mechanism of TodS that is activated in response to toluene. The crystal structures of the TodS Per-Arnt-Sim (PAS) 1 sensor domain (residues 43-164) and its complex with toluene (agonist) or 1,2,4-trimethylbenzene (antagonist) show a typical β2α3β3 PAS fold structure (residues 45-149), forming a hydrophobic ligand-binding site. A signal transfer region (residues 150-163) located immediately after the canonical PAS fold may be intrinsically flexible and disordered in both apo-PAS1 and antagonist-bound forms and dramatically adapt an α-helix upon toluene binding. This structural change in the signal transfer region is proposed to result in signal transmission to activate the TodS/TodT two-component signal transduction system. Site-directed mutagenesis and β-galactosidase assays using a P. putida reporter strain system verified the essential residues involved in ligand sensing and signal transfer and suggest that the Phe(46) residue acts as a ligand-sp...Continue Reading

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Citations

Oct 21, 2016·Microbial Biotechnology·Verónica Hernández-SánchezAna Segura
Mar 2, 2021·Current Opinion in Microbiology·Erwin C StuffleKylie J Watts
Apr 5, 2021·Environmental Science and Pollution Research International·Ma Laura Ortiz-HernándezPatricia Mussali-Galante

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Methods Mentioned

BETA
PCR
size exclusion chromatography
isothermal titration calorimetry
light
transmission electron microscopy
x-ray diffraction
static light scattering
light scattering
PISA
electron microscopy

Software Mentioned

Origin
MOLREP
COOT
PISA
AutoBuild
AutoSol
HKL2000
MicroCal
PHENIX
Astra

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