Molecular insights into Vibrio cholerae's intra-amoebal host-pathogen interactions.

Nature Communications
Charles Van der HenstMelanie Blokesch

Abstract

Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonly found in aquatic habitats. Within such environments, the bacterium must defend itself against predatory protozoan grazers. Amoebae are prominent grazers, with Acanthamoeba castellanii being one of the best-studied aquatic amoebae. We previously showed that V. cholerae resists digestion by A. castellanii and establishes a replication niche within the host's osmoregulatory organelle. In this study, we decipher the molecular mechanisms involved in the maintenance of V. cholerae's intra-amoebal replication niche and its ultimate escape from the succumbed host. We demonstrate that minor virulence features important for disease in mammals, such as extracellular enzymes and flagellum-based motility, have a key role in the replication and transmission of V. cholerae in its aqueous environment. This work, therefore, describes new mechanisms that provide the pathogen with a fitness advantage in its primary habitat, which may have contributed to the emergence of these minor virulence factors in the species V. cholerae.

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Citations

Feb 15, 2019·Environmental Microbiology·Lisa C MetzgerMelanie Blokesch
May 5, 2020·Environmental Microbiology·Delphine Destoumieux-GarzónLuigi Vezzulli
Sep 5, 2020·Environmental Microbiology·Natália C Drebes Dörr, Melanie Blokesch
Feb 11, 2020·Frontiers in Microbiology·Gustavo Espinoza-VergaraParisa Noorian
Oct 28, 2019·Experimental Parasitology·Sonali K KalraUjjala Ghoshal
Dec 17, 2020·Microorganisms·Richard GuillonneauMaëlle Molmeret
Apr 8, 2021·Current Biology : CB·Benjamin R WucherCarey D Nadell
Jun 8, 2021·Environmental Microbiology·Aide LasaLuigi Vezzulli

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Methods Mentioned

BETA
transmission electron microscopy
electron microscopy
confocal microscopy
motility assays
PCR
reverse transcription PCR
scanning electron microscopy

Software Mentioned

ImageJ
GraphPad
FIJI
Blender
TrakEM2
Adobe
Photoshop
GraphPad Prism

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