Molecular integration of wingless, decapentaplegic, and autoregulatory inputs into Distalless during Drosophila leg development

Developmental Cell
Carlos EstellaRichard S Mann

Abstract

The development of the Drosophila leg requires both Decapentaplegic (Dpp) and Wingless (Wg), two signals that establish the proximo-distal (PD) axis by activating target genes such as Distalless (Dll). Dll expression in the leg depends on a Dpp- and Wg-dependent phase and a maintenance phase that is independent of these signals. Here, we show that accurate Dll expression in the leg results from the synergistic interaction between two cis-regulatory elements. The Leg Trigger (LT) element directly integrates Wg and Dpp inputs and is only active in cells receiving high levels of both signals. The Maintenance (M) element is able to maintain Wg- and Dpp-independent expression, but only when in cis to LT. M, which includes the native Dll promoter, functions as an autoregulatory element by directly binding Dll. The "trigger-maintenance" model describes a mechanism by which secreted morphogens act combinatorially to induce the stable expression of target genes.

References

Mar 1, 1991·Mechanisms of Development·B CohenS M Cohen
Feb 26, 1993·Cell·G Struhl, K Basler
May 15, 1996·Genes & Development·C MerliR K Blackman
Dec 15, 1996·Genes & Development·N AzpiazuM Frasch
Jul 10, 1997·Nature·T Lecuit, S M Cohen
Mar 23, 2001·The Journal of Biological Chemistry·H KirkpatrickA Laughon
Aug 9, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L Sánchez, I Guerrero
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Vincent C CalhounMichael Levine
Aug 30, 2002·Current Opinion in Genetics & Development·Richard S Mann, Sean B Carroll
Sep 13, 2002·Insect Biochemistry and Molecular Biology·D N Arnosti
Oct 17, 2002·Genes & Development·Jennifer E F Butler, James T Kadonaga
Jun 28, 2003·Methods : a Companion to Methods in Enzymology·Young Sik Lee, Richard W Carthew
Dec 1, 2004·Annual Review of Genetics·Leonie Ringrose, Renato Paro
Feb 11, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hugh W Brock, Cynthia L Fisher
Mar 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sorin Istrail, Eric H Davidson
Mar 7, 2007·International Review of Cytology·Achim BreilingValerio Orlando
Jan 11, 2008·Development·Carlos Estella, Richard S Mann

❮ Previous
Next ❯

Citations

Dec 22, 2009·Development Genes and Evolution·Daniela GrossmannNikola-Michael Prpic
Aug 28, 2013·Apoptosis : an International Journal on Programmed Cell Death·Sol FereresAna Busturia
Aug 29, 2012·Genes & Development·Raghavendra NagarajUtpal Banerjee
Oct 13, 2011·Science Signaling·Edward EiversEdward M De Robertis
Jan 11, 2008·Development·Carlos Estella, Richard S Mann
Jan 7, 2011·Development·Javier MoranteClaude Desplan
Feb 12, 2015·Developmental Cell·Daniel J McKayA Gregory Matera
Sep 14, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nicolás Frankel
Sep 3, 2011·EMBO Reports·Andrés Dekanty, Marco Milán
Jun 23, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nobue Itasaki, Stefan Hoppler
Feb 4, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Cora BergantiñosFlorenci Serras
Nov 21, 2012·Developmental Biology·Thomas IngGerold Schubiger
Aug 28, 2012·Developmental Biology·Daniela Grossmann, Nikola-Michael Prpic
Apr 12, 2008·Current Biology : CB·Javier Morante, Claude Desplan
Jul 23, 2013·Gene Expression Patterns : GEP·Sandra Treffkorn, Georg Mayer
Mar 18, 2009·Developmental Cell·Mary Y Wu, Caroline S Hill
Apr 24, 2012·Cell Reports·Marios AgelopoulosRichard S Mann
Nov 16, 2013·Developmental Cell·Daniel J McKay, Jason D Lieb
Feb 16, 2011·Developmental Biology·Máximo Ibo GalindoJuan Pablo Couso
May 18, 2011·Developmental Biology·Youngwook AhnPamela J Mitchell
Feb 20, 2015·PLoS Genetics·Carla S Lopes, Fernando Casares
Feb 18, 2017·ELife·Olivia SchwarzJan Pielage
Mar 16, 2017·ELife·Laurens M van TienenMariann Bienz
Dec 4, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Francisco HuizarJeremiah Zartman
Jun 7, 2019·Nature Communications·Ilenia BernasconeFernando Martín-Belmonte

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.