Molecular interaction studies of trimethoxy flavone with human serum albumin.

PloS One
Mahesh GokaraRajagopal Subramanyam

Abstract

Human serum albumin (HSA) is the most abundant protein in blood plasma, having high affinity binding sites for several endogenous and exogenous compounds. Trimethoxy flavone (TMF) is a naturally occurring flavone isolated from Andrographis viscosula and used in the treatment of dyspepsia, influenza, malaria, respiratory functions and as an astringent and antidote for poisonous stings of some insects. The main aim of the experiment was to examine the interaction between TMF and HSA at physiological conditions. Upon addition of TMF to HSA, the fluorescence emission was quenched and the binding constant of TMF with HSA was found to be K(TMF) = 1.0+/-0.01x10(3) M(-1), which corresponds to -5.4 kcal M(-1) of free energy. Micro-TOF Q mass spectrometry results showed a mass increase of from 66,513 Da (free HSA) to 66,823 Da (HAS +Drug), indicating the strong binding of TMF with HSA resulting in decrease of fluorescence. The HSA conformation was altered upon binding of TMF to HSA with decrease in alpha-helix and an increase in beta-sheets and random coils suggesting partial unfolding of protein secondary structure. Molecular docking experiments found that TMF binds strongly with HSA at IIIA domain of hydrophobic pocket with hydrogen bo...Continue Reading

References

Jul 16, 1992·Nature·X M He, D C Carter
Aug 30, 1985·Biochemical and Biophysical Research Communications·A K Srivastava
Dec 15, 1994·European Journal of Biochemistry·D C CarterZ Krishnasami
Oct 28, 1994·Biochemical and Biophysical Research Communications·V Sanchez-MargáletC K Sung
Dec 1, 1995·Journal of Computer-aided Molecular Design·G JonesR C Glen
Apr 4, 1997·Journal of Molecular Biology·G JonesR Taylor
Jul 2, 1999·Protein Engineering·S SugioK Kobayashi
Nov 26, 1999·Biochimica Et Biophysica Acta·S CurryN P Franks
Apr 4, 2001·The Journal of Biological Chemistry·I PetitpasS Curry
May 26, 2001·Annual Review of Nutrition·C S YangH L Newmark
Nov 26, 2002·Biochemical and Biophysical Research Communications·Bidisa Sengupta, Pradeep K Sengupta
Apr 24, 2003·Medicinal Research Reviews·Wenying RenLi Zhang
Aug 12, 2003·Proteins·Marcel L VerdonkRichard D Taylor
Dec 4, 2003·Chemical & Pharmaceutical Bulletin·Yerra Koteswara RaoTian-Shung Wu
Nov 24, 2004·International Journal of Biological Macromolecules·Qingquan BianZhide Hu
Sep 20, 2005·Journal of Molecular Biology·Jamie GhumanStephen Curry
Nov 8, 2005·Toxicology·Amit Chaudhary, Kristine L Willett
Dec 23, 2006·International Journal of Biological Macromolecules·C N N'soukpoé-KossiH A Tajmir-Riahi
Jan 16, 2007·Biochemical Pharmacology·Elena Karnaukhova
Jul 3, 2007·European Journal of Medicinal Chemistry·Ping Yi, Minghua Qiu
Sep 25, 2007·Biomacromolecules·R BeaucheminH A Tajmir-Riahi
Dec 11, 2007·Biomacromolecules·Li LiangMuriel Subirade
Mar 4, 2008·Journal of Pharmaceutical and Biomedical Analysis·P B KandagalJ Seetharamappa
Apr 5, 2008·The Journal of Physical Chemistry. B·Sudarson Sekhar SinhaSamir Kumar Pal
Apr 18, 2008·Analytical and Bioanalytical Chemistry·Andréa DinizMaría José Medina-Hernández
Oct 24, 2008·Journal of Photochemistry and Photobiology. B, Biology·Rajagopal SubramanyamDamu G Amooru
Jan 16, 2009·The Journal of Physical Chemistry. B·David CharbonneauHeidar-Ali Tajmir-Riahi
Jan 23, 2009·Journal of Pharmaceutical and Biomedical Analysis·Yuanyuan YueXiaojun Yao
Feb 24, 2009·Journal of Photochemistry and Photobiology. B, Biology·Rajagopal SubramanyamDamu G Amooru
Mar 26, 2009·Chirality·Ankita VarshneyRizwan Hasan Khan
Apr 23, 2009·The Journal of Physical Chemistry. B·E FroehlichH A Tajmir-Riahi

❮ Previous
Next ❯

Citations

Oct 12, 2013·Apoptosis : an International Journal on Programmed Cell Death·Madhavi Gorla, Naresh Babu V Sepuri
Mar 4, 2014·International Journal of Molecular Sciences·Feng YangHong Liang
Sep 12, 2014·Molecular BioSystems·Daniel Pushparaju Yeggoni, Rajagopal Subramanyam
Aug 27, 2013·Journal of Agricultural and Food Chemistry·Xuli WuJijuan Cao
Nov 15, 2011·Journal of Molecular Modeling·Chandramouli MalledaRajagopal Subramanyam
Feb 19, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Feng YangHong Liang
Jul 4, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·F Janati FardM Javan Khoshkholgh
Feb 7, 2012·Chemosphere·Asma Yasmeen KhanGopinatha Suresh Kumar
Oct 18, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·W SongL J Yu
Aug 8, 2013·Journal of Biochemical and Molecular Toxicology·Junlong WuWeihua Ren
Nov 22, 2012·Journal of Biochemical and Molecular Toxicology·Jiongwei PanZhuo Cao
Dec 12, 2013·Chemical Biology & Drug Design·Mei LiHong Liang
Dec 24, 2013·Journal of Biomolecular Structure & Dynamics·Mahesh GokaraRajagopal Subramanyam
Feb 14, 2013·International Journal of Biological Macromolecules·Hongliang LiJianke Li
Jul 24, 2014·Journal of Biomolecular Structure & Dynamics·Daniel Pushparaju YeggoniRajagopal Subramanyam
Jul 13, 2013·Journal of Biomolecular Structure & Dynamics·Mahesh GokaraRajagopal Subramanyam
Oct 7, 2015·Journal of Biomolecular Structure & Dynamics·Daniel Pushpa Raju YeggoniRajagopal Subramanyam
Dec 9, 2015·Journal of Molecular Recognition : JMR·T V M SreekanthYong Rok Lee
Aug 27, 2016·Journal of Biomolecular Structure & Dynamics·Mohammad Hossein MehrabanYounes Ghasemi
Nov 3, 2016·Luminescence : the Journal of Biological and Chemical Luminescence·Xin GaoLin Tang
Feb 25, 2015·Molecular BioSystems·Monika KallubaiRajagopal Subramanyam
May 18, 2019·Journal of Biomolecular Structure & Dynamics·Shreya DubeyRajagopal Subramanyam

❮ Previous
Next ❯

Methods Mentioned

BETA
circular dichroism
column chromatography

Software Mentioned

SILVERv1
InsightII
CDNN
GOLDv3
GOLD ( Genetic Optimization for Ligand Docking )
/ Builder
Bruker analysis

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.