Molecular interactions between Geobacter sulfurreducens triheme cytochromes and the redox active analogue for humic substances

Biochimica Et Biophysica Acta. Bioenergetics
Joana M DantasCarlos A Salgueiro

Abstract

The bacterium Geobacter sulfurreducens can transfer electrons to quinone moieties of humic substances or to anthraquinone-2,6-disulfonate (AQDS), a model for the humic acids. The reduced form of AQDS (AH2QDS) can also be used as energy source by G. sulfurreducens. Such bidirectional utilization of humic substances confers competitive advantages to these bacteria in Fe(III) enriched environments. Previous studies have shown that the triheme cytochrome PpcA from G. sulfurreducens has a bifunctional behavior toward the humic substance analogue. It can reduce AQDS but the protein can also be reduced by AH2QDS. Using stopped-flow kinetic measurements we were able to demonstrate that other periplasmic members of the PpcA-family in G. sulfurreducens (PpcB, PpcD and PpcE) also showed the same behavior. The extent of the electron transfer is thermodynamically controlled favoring the reduction of the cytochromes. NMR spectra recorded for 13C,15N-enriched samples in the presence increasing amounts of AQDS showed perturbations in the chemical shift signals of the cytochromes. The chemical shift perturbations on cytochromes backbone NH and 1H heme methyl signals were used to map their interaction regions with AQDS, showing that each protein...Continue Reading

References

Sep 1, 1995·Journal of Biomolecular NMR·D S WishartB D Sykes
Oct 29, 1998·Biochemical and Biophysical Research Communications·E ArslanL Thöny-Meyer
Feb 24, 2001·Environmental Microbiology·D R LovleyE L Blunt-Harris
Mar 2, 2002·Journal of Bacteriology·J Bruce H ShyuDianne K Newman
Mar 7, 2003·Applied and Environmental Microbiology·Daniel R Bond, Derek R Lovley
Apr 25, 2003·The Science of the Total Environment·Jie ChenWilliam D Burgos
Nov 3, 2004·Advances in Microbial Physiology·Derek R LovleyKelly P Nevin
Jul 22, 2005·Photosynthesis Research·Marcellus Ubbink
Dec 6, 2005·Environmental Science & Technology·Kelvin B Gregory, Derek R Lovley
Sep 19, 2006·Nature Reviews. Microbiology·Karrie A WeberJohn D Coates
Oct 12, 2007·Biotechnology and Bioengineering·Shweta SrikanthDaniel R Bond
Oct 24, 2007·Journal of Biomolecular NMR·Frank H SchumannHans Robert Kalbitzer
Mar 18, 2008·Protein Expression and Purification·Ana P FernandesCarlos A Salgueiro
Jul 22, 2008·Biochimica Et Biophysica Acta·Yan-Huai R DingTünde Mester
Oct 14, 2008·Applied and Environmental Microbiology·Enrico MarsiliDaniel R Bond
Dec 7, 2010·Nature Reviews. Microbiology·Radhakrishnan MahadevanDerek R Lovley
Mar 1, 2011·The FEBS Journal·Qamar BashirMarcellus Ubbink
May 13, 2011·Journal of the American Chemical Society·Kimberley R Cousins
Jun 15, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ying LiuDaniel R Bond
Oct 21, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Joana M DantasRicardo O Louro
Nov 26, 2011·Advances in Microbial Physiology·Derek R LovleyKelly P Nevin
Apr 22, 2015·Biochimica Et Biophysica Acta·Zhu LiuChun Tang
Apr 24, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Telma C SantosCarlos A Salgueiro

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.