Molecular Interactions between MUC1 Epithelial Mucin, β-Catenin, and CagA Proteins.

Frontiers in Immunology
Wei GuangErik P Lillehoj

Abstract

Interleukin (IL)-8-driven neutrophil infiltration of the gastric mucosa is pathognomonic of persistent Helicobacter pylori infection. Our prior study showed that ectopic over-expression of MUC1 in human AGS gastric epithelial cells reduced H. pylori-stimulated IL-8 production compared with cells expressing MUC1 endogenously. Conversely, Muc1 knockout (Muc1(-/-)) mice displayed an increased level of transcripts encoding the keratinocyte chemoattractant (KC), the murine equivalent of human IL-8, in gastric mucosa compared with Muc1(+/+) mice during experimental H. pylori infection. The current study tested the hypothesis that a decreased IL-8 level observed following MUC1 over-expression is mediated through the ability of MUC1 to associate with β-catenin, thereby inhibiting H. pylori-induced β-catenin nuclear translocation. Increased neutrophil infiltration of the gastric mucosa of H. pylori-infected Muc1(-/-) mice was observed compared with Muc1(+/+) wild type littermates, thus defining the functional consequences of increased KC expression in the Muc1-null animals. Protein co-immunoprecipitation (co-IP) studies using lysates of untreated or H. pylori-treated AGS cells demonstrated that (a) MUC1 formed a co-IP complex with β-cat...Continue Reading

Citations

May 9, 2014·International Journal of Molecular Sciences·Norihisa SaekiTeruhiko Yoshida
Dec 1, 2017·Journal of Clinical Medicine·Kosuke KatoKwang Chul Kim
Aug 22, 2015·Cellular and Molecular Life Sciences : CMLS·Vasso ApostolopoulosSharron E Gargosky
May 18, 2017·Frontiers in Medicine·Claudia I Rivas-OrtizGonzalo Castillo-Rojas
Mar 5, 2016·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Tessa HoremansPaul Cos

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Methods Mentioned

BETA
co-immunoprecipitation
immunoprecipitation
co-IP
confocal microscopy
co-IPs
nuclear translocation

Software Mentioned

Adobe Photoshop

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