PMID: 3762594Sep 1, 1986Paper

Molecular karyotype of species and subspecies of Leishmania

Molecular and Biochemical Parasitology
J K SchollerK Stuart

Abstract

The DNA karyotypes of three species and several subspecies of New World Leishmania were found to be distinct. The karyotypes were more similar among closely related isolates than among more distantly related groups. Two classes of chromosomal DNA differences were detected among stocks; +/- 50 kb size differences among DNAs, some of which were shown to contain homologous sequences, and DNAs having no obvious corresponding chromosomal DNA in other isolates. A total of 14-24 chromosomal DNA bands were resolved, depending on the isolate, but densitometric analyses suggest that these isolates contain 26-33 distinct DNA molecules. These molecules total about 2.5 X 10(7) bp, a substantial fraction of the genomic DNA. The chromosomal DNA locations of gene sequences homologous to alpha- and beta-tubulin, ribosomal RNA, thymidylate synthetase-dihydrofolate reductase, and the H-region sequence were determined. The homologous sequences were located on chromosomal DNAs of similar, but not identical sizes among different stocks. We also found species- and some subspecies-specific beta-tubulin chromosomal loci. We conclude that the DNA karyotype is useful for stock identification, taxonomy, and gene localization in Leishmania. Its potential f...Continue Reading

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Citations

Sep 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·R P Bishop, F Akinsehinwa
Jul 1, 1990·Transactions of the Royal Society of Tropical Medicine and Hygiene·J ValdésE Orozco
Jan 15, 1987·Molecular and Biochemical Parasitology·D P JasmerK Stuart
Oct 1, 1987·Molecular and Biochemical Parasitology·N Samaras, T W Spithill
Jun 1, 1988·Molecular and Biochemical Parasitology·P J MylerK D Stuart
May 1, 1988·Molecular and Biochemical Parasitology·J Ellis, J Crampton
Oct 1, 1988·Molecular and Biochemical Parasitology·D Fong, B Lee
May 15, 1989·Molecular and Biochemical Parasitology·I Galindo, J L Ramírez Ochoa
Jun 1, 1989·Molecular and Biochemical Parasitology·J K SchollerK D Stuart
Feb 1, 1990·Molecular and Biochemical Parasitology·S H GianniniL H Van der Ploeg
Mar 1, 1990·Molecular and Biochemical Parasitology·R A MillerM Parsons
Aug 1, 1990·Molecular and Biochemical Parasitology·B L SmileyK Stuart
Jan 1, 1990·Molecular and Biochemical Parasitology·R P Bishop
Mar 1, 1991·Molecular and Biochemical Parasitology·J C MeadeJ R Stringer
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Jul 28, 2004·Parasitology Today·M Segovia, G Ortiz

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