Molecular keys of the tropism of integration of the cholera toxin phage.

Proceedings of the National Academy of Sciences of the United States of America
Bhabatosh DasFrançois-Xavier Barre

Abstract

Cholera toxin is encoded in the genome of CTXvarphi, a lysogenic filamentous phage of Vibrio cholerae. CTXvarphi variants contribute to the genetic diversity of cholera epidemic strains. It has been shown that the El Tor variant of CTXvarphi hijacks XerC and XerD, two host-encoded tyrosine recombinases that normally function to resolve chromosome dimers, to integrate at dif1, the dimer resolution site of the larger of the two V. cholerae chromosomes. However, the exact mechanism of integration of CTXvarphi and the rules governing its integration remained puzzling, with phage variants integrated at either or both dimer resolution sites of the two V. cholerae chromosomes. We designed a genetic system to determine experimentally the tropism of integration of CTXvarphi and thus define rules of compatibility between phage variants and dimer resolution sites. We then showed in vitro how these rules are explained by the direct integration of the single-stranded phage genome into the double-stranded bacterial genome. Finally, we showed how the evolution of phage attachment and chromosome dimer resolution sites contributes to the generation of genetic diversity among cholera epidemic strains.

References

Mar 11, 1986·Nucleic Acids Research·R H HoessK Abremski
May 12, 1995·The Journal of Biological Chemistry·X D ZhuP D Sadowski
Jan 1, 1995·Clinical Microbiology Reviews·J B KaperM M Levine
Feb 22, 1996·Gene·K Skorupski, R K Taylor
Jun 28, 1996·Science·M K Waldor, J J Mekalanos
Oct 31, 1997·Journal of Molecular Biology·S E Nunes-DübyA Landy
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·B M Davis, M K Waldor
Jun 7, 2002·Nature·Kathryn E Huber, Matthew K Waldor
Apr 28, 2004·Plasmid·Nadège PhilippeDominique Schneider
Sep 15, 2004·Journal of Molecular Biology·Sang Yeol Lee, Arthur Landy
Nov 4, 2004·Molecular Microbiology·Sarah M McLeod, Matthew K Waldor
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Deborah T Hung, John J Mekalanos
Apr 28, 2006·Nature·Douglas MacDonaldDeshmukh N Gopaul
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Shah M FaruqueG Balakrish Nair
Aug 28, 2007·Nucleic Acids Research·Karolina MalanowskaJeffrey F Gardner
Aug 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·S M Nashir UddenShah M Faruque
Sep 27, 2008·PLoS Genetics·Marie-Eve ValFrançois-Xavier Barre
Jan 1, 2009·Emerging Infectious Diseases·Amit RaychoudhuriAsish K Mukhopadhyay
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jongsik ChunRita R Colwell

❮ Previous
Next ❯

Citations

Jun 5, 2012·Molecular Genetics and Genomics : MGG·Ahmed AskoraTakashi Yamada
Mar 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·E Fidelma Boyd
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Bhabatosh DasFrançois-Xavier Barre
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·David BikardDidier Mazel
Nov 7, 2012·Trends in Microbiology·Bhabatosh DasFrançois-Xavier Barre
Dec 7, 2013·Molecular Microbiology·Marie-Eve ValDidier Mazel
Feb 14, 2015·Annals of the New York Academy of Sciences·Mart Krupovic, Patrick Forterre
Apr 2, 2016·Journal of Microbiological Methods·Mario Juhas, James W Ajioka
Jan 1, 2013·Bacteriophage·E Fidelma BoydNityananda Chowdhury
Oct 19, 2012·Virulence·Shah M Faruque, John J Mekalanos
Jan 28, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rachana BanerjeeSurajit Basak
Dec 17, 2014·Frontiers in Microbiology·Bhabatosh Das
Nov 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Caroline MidonetFrancois-Xavier Barre
May 21, 2015·PLoS Genetics·Eriel MartínezFrançois-Xavier Barre
Jul 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Caroline Midonet, François-Xavier Barre
Oct 28, 2015·Journal of Bacteriology·Archana PantBhabatosh Das
Sep 17, 2014·Journal of Bacteriology·Bhabatosh DasGopinath Balakrish Nair
Apr 7, 2019·EMBO Reports·Iain D Hay, Trevor Lithgow
Aug 24, 2019·Proceedings of the National Academy of Sciences of the United States of America·Bhabatosh Das
Aug 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Caroline MidonetFrançois-Xavier Barre
Aug 19, 2016·Current Opinion in Infectious Diseases·Bhabatosh DasThandavarayan Ramamurthy
Mar 23, 2018·Frontiers in Microbiology·Hyun J YuDong W Kim
Feb 2, 2018·Physical Chemistry Chemical Physics : PCCP·Carolyn E Carr, Luis A Marky
Sep 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Archana PantBhabatosh Das
Jun 24, 2018·Frontiers of Medicine·Ali A Rabaan
Oct 31, 2020·Frontiers in Cellular and Infection Microbiology·Ashok KumarNiraj Kumar
Aug 10, 2017·Biophysical Journal·Carolyn E Carr, Luis A Marky
Jun 25, 2015·Microbiology Spectrum·Caroline Midonet, Francois-Xavier Barre
Sep 28, 2021·Frontiers in Microbiology·Imchang LeeJongsik Chun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.