Molecular level responses to chronic versus pulse nutrient loading in the seagrass Posidonia oceanica undergoing herbivore pressure

Oecologia
Miriam RuoccoGabriele Procaccini

Abstract

Seagrasses are key marine foundation species, currently declining due to the compounded action of global and regional anthropogenic stressors. Eutrophication has been associated with seagrass decline, while grazing has been traditionally considered to be a natural disturbance with a relatively low impact on seagrasses. In the recent years, this assumption has been revisited. Here, by means of a 16-month field-experiment, we investigated the molecular mechanisms driving the long-term response of Posidonia oceanica to the combination of nutrient enrichment, either as a chronic (press) or pulse disturbance, and herbivory. Changes in expression levels of 19 target genes involved in key steps of photosynthesis, nutrient assimilation, chlorophyll metabolism, oxidative-stress response and plant defense were evaluated through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). High herbivore pressure affected the molecular response of P. oceanica more dramatically than did enhanced nutrient levels, altering the expression of genes involved in plant tolerance and resistance traits, such as photosynthesis and defense mechanisms. Genes involved in carbon fixation and N assimilation modulated the response of plants to h...Continue Reading

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Citations

Dec 19, 2019·Frontiers in Plant Science·Miriam RuoccoGabriele Procaccini
May 25, 2021·Evolutionary Applications·Jessica PazzagliaGabriele Procaccini

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Datasets Mentioned

BETA
GEMD01054723.1
KC954696

Methods Mentioned

BETA
electrophoresis
PCR
PCA

Software Mentioned

PERMANOVA
NormFinder
geNorm
Primer
BestKeeper
STATISTICA
PAST

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