Molecular magnetic resonance imaging of angiogenesis in vivo using polyvalent cyclic RGD-iron oxide microparticle conjugates

Theranostics
Stavros MelemenidisRobin P Choudhury

Abstract

Angiogenesis is an essential component of tumour growth and, consequently, an important target both therapeutically and diagnostically. The cell adhesion molecule α(v)β(3) integrin is a specific marker of angiogenic vessels and the most prevalent vascular integrin that binds the amino acid sequence arginine-glycine-aspartic acid (RGD). Previous studies using RGD-targeted nanoparticles (20-50 nm diameter) of iron oxide (NPIO) for magnetic resonance imaging (MRI) of tumour angiogenesis, have identified a number of limitations, including non-specific extravasation, long blood half-life (reducing specific contrast) and low targeting valency. The aim of this study, therefore, was to determine whether conjugation of a cyclic RGD variant [c(RGDyK)], with enhanced affinity for α(v)β(3), to microparticles of iron oxide (MPIO) would provide a more sensitive contrast agent for imaging of angiogenic tumour vessels. Cyclic RGD [c(RGDyK)] and RAD [c(RADyK)] based peptides were coupled to 2.8 μm MPIO, and binding efficacy tested both in vitro and in vivo. Significantly greater specific binding of c(RGDyK)-MPIO to S-nitroso-n-acetylpenicillamine (SNAP)-stimulated human umbilical vein endothelial cells in vitro than PBS-treated cells was demons...Continue Reading

Citations

Sep 30, 2016·Current Cardiovascular Imaging Reports·Ljubica MandicMariann Gyöngyösi
Nov 1, 2017·European Journal of Nuclear Medicine and Molecular Imaging·Weijun WeiWeibo Cai
May 19, 2018·Organic & Biomolecular Chemistry·Fabien ThoreauDidier Boturyn
Oct 21, 2015·Magnetic Resonance Insights·Daniel J KorchinskiJeff F Dunn
Jul 5, 2018·Advanced Materials·Zheyu ShenAiguo Wu
Apr 30, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Xiaowei Wang, Karlheinz Peter
Oct 11, 2019·Biomolecules & Therapeutics·Joon-Seok Choi, Sang Hoon Joo
Jun 27, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Caroline J ThébaultBich-Thuy Doan
Sep 9, 2018·Proceedings of the National Academy of Sciences of the United States of America·Evgenii L GuryevSergey M Deyev
May 24, 2017·Proceedings of the National Academy of Sciences of the United States of America·Antoine Philippe FournierRichard Macrez
Jan 28, 2021·International Journal of Molecular Sciences·Si-Yen LiuChih-Hsien Chang
Jan 11, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Liron L IsraelJulia Y Ljubimova
Sep 27, 2020·Biomaterials·Nisakorn YodsanitShaoqin Gong
Jul 24, 2021·British Journal of Pharmacology·Xiaowei WangKarlheinz Peter

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
xenograft

Software Mentioned

MPIO
ImagePro Plus
Image Scope
MATLAB
Snap
ITK

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.