Molecular mapping of the conductance activity linked to tAE1 expressed in Xenopus oocyte

Biochimica Et Biophysica Acta
Franck BorgeseH Guizouarn

Abstract

It was previously shown that expressed in Xenopus oocyte the trout (tAE1) and the mouse (mAE1) anion exchangers behave differently: both elicit anion exchange activity but only tAE1 induces a transport of organic solutes correlated with an anion conductance. In order to identify the structural domains involved in the induction of tAE1 channel activity, chimeras have been prepared between mouse and trout AE1. As some constructs were not expressed at the plasma membrane, skate exchanger (skAE1) was used instead of mouse exchanger to complete the structure-function analysis. The present paper shows that skAE1, highly similar to mAE1, does not induce a chloride conductance when expressed in Xenopus oocyte. Construct expression analysis showed that only tAE1 transmembrane domain is linked to the anion conductance. More precisely, we identified two regions composed of helices 6, 7 and 8 and putative helices 12 and 13 which are required for this function.

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Citations

Oct 30, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J C ElloryG W Stewart
May 19, 2009·The Journal of Experimental Biology·Seth L Alper
May 29, 2007·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·C H Tang, T H Lee
May 24, 2007·Journal of Cellular Physiology·Sonia MartialFranck Borgese
Sep 10, 2005·Biochimica Et Biophysica Acta·Hélène GuizouarnFranck Borgese
Jan 7, 2018·Pflügers Archiv : European journal of physiology·Friederike Stumpff
May 26, 2005·American Journal of Physiology. Renal Physiology·Boris E ShmuklerSeth L Alper
Feb 24, 2007·The Journal of Physiology·Mark D ParkerMichael J A Tanner

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