Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust

PloS One
Jie ZhangPu Xuan

Abstract

Dasypyrum villosum has been used as a valuable gene resource for disease resistances, yield increase and quality improvement in wheat. A novel wheat-D. villosum alien introgression line CD-3 was generated through hybridization between the common wheat Chinese Spring (CS) and a CS- D. villosum 3V addition line having considerably high stripe rust resistance, which enable the characterization of a potential new stripe rust resistance gene (s) derived from D. villosum. The results of non-denaturing fluorescent in situ hybridization (ND-FISH) showed that CD-3 contained 42 chromosomes, including a 3V chromosome pair, and the absence of both of the 3D chromosomes. PCR-based Landmark Unique Gene (PLUG) molecular marker analysis supported results from the FISH analysis, revealing CD-3 was a wheat-D. villosum 3V (3D) disomic substitution line. Resistant test of stripe rust on 52 plants of F2 generation (CD-3/CS), CD-3, CS and D.villosum have been conducted at seedling stage. 7 plants of F2 generation possessing two 3V chromosomes exhibited high resistance to stripe rust as CD-3 and D.villosum, 10 plants carrying one 3V chromosome and 35 plants without 3V chromosome were susceptive to stripe rust as CS. The result implied the high stripe...Continue Reading

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Jul 27, 1999·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A G AlkhimovaA V Vershinin
Sep 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Akio KatoJames A Birchler
Dec 6, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Goro IshikawaTakashi Matsumoto
Apr 13, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Miguel BentoManuela Silva
Sep 15, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Q LiJ X Jing
Apr 19, 2011·Trends in Plant Science·Rajeev K VarshneyPeter Q Craufurd
Aug 24, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Shulan FuFangpu Han
Nov 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P D ChenD J Liu
Apr 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S N SibikeevV A Krupnov
Sep 24, 2014·Cytogenetic and Genome Research·Guang-Rong LiZu-Jun Yang
May 23, 2015·Scientific Reports·Shulan FuZongxiang Tang
Sep 4, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ruiqi ZhangPeidu Chen
Jul 17, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ruiqi ZhangPeidu Chen
Jul 21, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhenzhen DongJorge Dubcovsky

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Methods Mentioned

BETA
PCR
chromosomal structural aberrations

Software Mentioned

Primer Premier

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