Molecular mechanism for establishment of signal-dependent regulation in the PhoP/PhoQ system.

The Journal of Biological Chemistry
Wei KongYixin Shi

Abstract

In this report, we demonstrate that H-NS is essential for establishing the Mg(2+)-responsive transcriptional regulation of the PhoP regulon in Salmonella. Deletion of this regulatory gene abolished the transcriptional repression of PhoP-activated genes when bacteria were grown in high environmental Mg(2+), thus stimulating expression of phoP and other PhoP regulon genes. In the absence of H-NS, transcriptional activation was PhoP-dependent for those genes only activated by PhoP, but was PhoP-independent for those genes activated by both PhoP and SlyA. The H-NS protein footprints the phoP promoter in a sequence located upstream of the PhoP box; mutation of this cis-acting factor abolished transcriptional repression of the phoP gene equivalent to the phenotype exhibited in the hns mutant. Further results showed that H-NS gel shifts other PhoP regulon promoters, indicating that a PhoP-activated gene would be transcriptionally repressed via direct H-NS binding and inhibition of its activator PhoP. Furthermore, H-NS footprints a newly identified SlyA box and the reverse PhoP box in the pagC promoter, suggesting that both SlyA and PhoP compete with this regulatory protein. Therefore, H-NS should pair with SlyA and PhoP to establish a...Continue Reading

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Citations

Dec 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Guang ZhaoYixin Shi
Sep 1, 2012·The Journal of Biological Chemistry·Nick QuadePetra Dersch
Aug 6, 2008·The Journal of Biological Chemistry·Haowei SongYixin Shi
Mar 13, 2012·Journal of Bacteriology·A BattestiS Gottesman
Apr 17, 2012·Journal of Bacteriology·Mar ReinésJosé A Bengoechea
Mar 23, 2012·Molecular Microbiology·Igor ZwirEduardo A Groisman
Oct 9, 2012·Computers in Biology and Medicine·Mahendra Kumar Prajapat, Supreet Saini
Feb 3, 2016·Infection and Immunity·Natasha Weatherspoon-Griffin, Helen J Wing
Mar 7, 2014·The Journal of Biological Chemistry·Yixin ShiWei Kong
May 25, 2021·Research in Microbiology·Fernanda Pereira da SilvaMaria Cristina Dantas Vanetti
Jul 1, 2021·Microbiology and Molecular Biology Reviews : MMBR·Eduardo A GroismanJeongjoon Choi

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