Molecular Mechanism for the Hofmeister Effect Derived from NMR and DSC Measurements on Barnase

ACS Omega
Jordan W ByeMike P Williamson

Abstract

The effects of sodium thiocyanate, sodium chloride, and sodium sulfate on the ribonuclease barnase were studied using differential scanning calorimetry (DSC) and NMR. Both measurements reveal specific and saturable binding at low anion concentrations (up to 250 mM), which produces localized conformational and energetic effects that are unrelated to the Hofmeister series. The binding of sulfate slows intramolecular motions, as revealed by peak broadening in 13C heteronuclear single quantum coherence spectroscopy. None of the anions shows significant binding to hydrophobic groups. Above 250 mM, the DSC results are consistent with the expected Hofmeister effects in that the chaotropic anion thiocyanate destabilizes barnase. In this higher concentration range, the anions have approximately linear effects on protein NMR chemical shifts, with no evidence for direct interaction of the anions with the protein surface. We conclude that the effects of the anions on barnase are mediated by solvent interactions. The results are not consistent with the predictions of the preferential interaction, preferential hydration, and excluded volume models commonly used to describe Hofmeister effects. Instead, they suggest that the Hofmeister anion e...Continue Reading

References

Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
Dec 7, 1982·Biochemistry·T Arakawa, S N Timasheff
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·M Jucovic, R W Hartley
Oct 1, 1996·Biophysical Journal·R L Baldwin
Jul 13, 2002·Biotechnology and Bioengineering·R A CurtisH W Blanch
Jul 19, 2003·Science·Anne Willem OmtaHuib J Bakker
Feb 20, 2004·Journal of the American Chemical Society·Joseph D BatchelorGary J Pielak
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bertil Halle
Oct 27, 2004·Biophysical Chemistry·Susanne MoelbertPaolo De Los Rios
Jan 18, 2005·International Journal of Pharmaceutics·Wei Wang
Sep 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Timothy O StreetGeorge D Rose
Nov 7, 2007·Nucleic Acids Research·Eldon L UlrichJohn L Markley
Nov 9, 2007·The Journal of Physical Chemistry. B·R MancinelliA K Soper
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Simon EbbinghausMartina Havenith
Feb 26, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Mikael LundPavel Jungwirth
Jun 3, 2008·Nucleic Acids Research·David J WiltonMike P Williamson
Dec 5, 1992·Biotechnology and Bioengineering·Y C ShihH W Blanch
Jul 18, 2008·The Journal of Physical Chemistry. B·Laurel M Pegram, M Thomas Record
Jan 20, 1997·Biotechnology and Bioengineering·K S Shiau, T L Chen
Jul 23, 2008·Physical Review Letters·Mikael LundClifford E Woodward
Aug 9, 2008·Journal of the American Chemical Society·Mikael LundPavel Jungwirth
Nov 4, 2008·Journal of Biomolecular NMR·Marina CioffiMike P Williamson
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yanjie Zhang, Paul S Cremer
Nov 4, 2009·Biophysical Journal·Xavier TadeoOscar Millet
Jan 9, 2010·Annual Review of Physical Chemistry·Yanjie Zhang, Paul S Cremer
Feb 10, 2010·Pharmaceutical Research·Mark Cornell ManningDerrick S Katayama
Apr 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Laurel M PegramM Thomas Record
May 22, 2010·Science·K J TielrooijH J Bakker
Jun 15, 2011·Physical Chemistry Chemical Physics : PCCP·Drew F ParsonsBarry W Ninham
Jan 19, 2012·Chemical Reviews·Pierandrea Lo Nostro, Barry W Ninham
Jun 13, 2012·Journal of the American Chemical Society·Kelvin B RembertPaul S Cremer
Jun 16, 2012·The Journal of Physical Chemistry. B·Jaibir KherbPaul S Cremer
Jun 6, 2013·Journal of the American Chemical Society·Blake M Rankin, Dor Ben-Amotz
Jun 26, 2013·Faraday Discussions·Santos Alexandre P dos, Yan Levin

❮ Previous
Next ❯

Citations

Jul 20, 2017·Physical Chemistry Chemical Physics : PCCP·Euihyun LeeMinhaeng Cho
Jan 17, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Olga A FranciscoMazdak Khajehpour
Apr 14, 2020·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Matja ZalarAlexander P Golovanov
Jun 25, 2021·Chemical Science·Wei YaoBruce C Gibb
Dec 29, 2018·The Journal of Physical Chemistry. B·Jordan W Bye, Robin A Curtis
Sep 24, 2021·Biophysical Journal·Phuong Thuy Bui, Trinh Xuan Hoang

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR

Software Mentioned

FELIX
naccess
Linux
NanoAnalyze
TopSpin

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.