Molecular mechanism of kNBC1-carbonic anhydrase II interaction in proximal tubule cells

The Journal of Physiology
Alexander PushkinIra Kurtz

Abstract

We have recently shown that carbonic anhydrase II (CAII) binds in vitro to the C-terminus of the electrogenic sodium bicarbonate cotransporter kNBC1 (kNBC1-ct). In the present study we determined the molecular mechanisms for the interaction between the two proteins and whether kNBC1 and CAII form a transport metabolon in vivo wherein bicarbonate is transferred from CAII directly to the cotransporter. Various residues in the C-terminus of kNBC1 were mutated and the effect of these mutations on both the magnitude of CAII binding and the function of kNBC1 expressed in mPCT cells was determined. Two clusters of acidic amino acids, L(958)DDV and D(986)NDD in the wild-type kNBC1-ct involved in CAII binding were identified. In both acidic clusters, the first aspartate residue played a more important role in CAII binding than others. A significant correlation between the magnitude of CAII binding and kNBC1-mediated flux was shown. The results indicated that CAII activity enhances flux through the cotransporter when the enzyme is bound to kNBC1. These data are the first direct evidence that a complex of an electrogenic sodium bicarbonate cotransporter with CAII functions as a transport metabolon.

References

Jul 1, 1977·Kidney International·T D McKinney, M B Burg
Oct 1, 1992·Pflügers Archiv : European journal of physiology·G Seki, E Frömter
Dec 1, 1985·Pflügers Archiv : European journal of physiology·K YoshitomiE Frömter
Aug 1, 1997·The Journal of Biological Chemistry·C E BurnhamM Soleimani
Oct 17, 1998·The Journal of Biological Chemistry·J W Vince, R A Reithmeier
Apr 23, 1999·The Journal of Biological Chemistry·E W MilesD R Davies
Dec 30, 2000·The Journal of Biological Chemistry·D BiemesderferP S Aronson
May 19, 2001·Blood Cells, Molecules & Diseases·R A Reithmeier
Jul 26, 2002·The Journal of Biological Chemistry·Xiuju LiLarry Fliegel
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Deborah SterlingJoseph R Casey
Jan 23, 2004·American Journal of Physiology. Cell Physiology·Frederick B LoiselleJoseph R Casey

❮ Previous
Next ❯

Citations

Mar 20, 2010·The Journal of Membrane Biology·Holger M BeckerJoachim W Deitmer
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Holger M BeckerJoachim W Deitmer
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kensuke NakajimaYusuke Matsuda
Nov 29, 2011·Brain : a Journal of Neurology·Thomas M BosleyKhaled K Abu-Amero
Aug 21, 2009·American Journal of Physiology. Cell Physiology·Joseph R CaseyBernardo V Alvarez
Dec 17, 2005·Critical Care : the Official Journal of the Critical Care Forum·Troels RingSøren Nielsen
May 19, 2009·The Journal of Experimental Biology·Walter F BoronMark D Parker
Mar 28, 2012·The Journal of Physiology·Malin H StridhHolger M Becker
Dec 14, 2006·Kidney International·J M Purkerson, G J Schwartz
Nov 3, 2009·Biochimica Et Biophysica Acta·Walter F Boron
Oct 31, 2006·Seminars in Nephrology·Angelito A BernardoJose A L Arruda
Oct 2, 2014·IUBMB Life·Kumari Alka, Joseph R Casey
Jun 19, 2012·Biochimica Et Biophysica Acta·Trevor F Moraes, Reinhart A F Reithmeier
Jan 19, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Riikka RiihonenTae-Hwan Kwon
Jul 1, 2015·Frontiers in Physiology·Ian M Thornell, Mark O Bevensee
Jan 23, 2015·Molecular Biology of the Cell·Gonzalo VilasRobert Todd Alexander
Mar 27, 2015·BioMed Research International·Melissa A PinardRobert McKenna
Jan 21, 2015·Frontiers in Neuroscience·Joachim W DeitmerHolger M Becker
Jul 23, 2014·BMC Cardiovascular Disorders·Daniel SowahJoseph R Casey
Oct 16, 2016·Plant, Cell & Environment·Michael GroszmannJohn R Evans
Aug 23, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nobuo TeradaShinichi Ohno
Mar 15, 2005·Molecular Membrane Biology·Patricio E MorganJoseph R Casey
Dec 4, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Solange Abdulnour-NakhoulRoy C Orlando
Oct 29, 2009·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Xavier CastellsCarles Arús
Jul 2, 2005·The EMBO Journal·Bernardo V AlvarezJoseph R Casey
Feb 8, 2006·American Journal of Physiology. Renal Physiology·Alexander Pushkin, Ira Kurtz
Apr 20, 2006·American Journal of Physiology. Renal Physiology·Doris Joy D EspirituJose A L Arruda
Jul 27, 2007·American Journal of Physiology. Cell Physiology·Patricio E MorganJoseph R Casey
Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron
May 6, 2011·American Journal of Physiology. Cell Physiology·Daniel Sowah, Joseph R Casey
Sep 22, 2005·The Journal of Biological Chemistry·Holger M BeckerJoachim W Deitmer
Apr 11, 2020·Cancers·Holger M Becker, Joachim W Deitmer
Jun 10, 2008·The Journal of Biological Chemistry·Holger M Becker, Joachim W Deitmer
Mar 14, 2007·The Journal of Biological Chemistry·Holger M Becker, Joachim W Deitmer
Jun 5, 2015·American Journal of Physiology. Renal Physiology·Devishree KrishnanR Todd Alexander
Dec 11, 2019·British Journal of Cancer·Holger M Becker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.