Mar 15, 2006

Molecular mechanism of myeloma cell line differentiation induced by 2-methoxyestradiol

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
Wei-Ran GaoHong Xiong

Abstract

To study the molecular mechanism of differentiation induction by 2-methoxyestradiol (2ME2) of myeloma cell lines. Differentiation induction effect on myeloma cell lines LP-1, CZ-1 and NCI-H929 which were incubated with 2ME2 and XBP-1, Blimp-1, pax-5 phosphorothioate antisense oligodeoxynucleotide (ASODN) was evaluated by cell morphology, CD49e expression, quantitation of light chain secretion, and the level of pax-5 and XBP-1 mRNA expression. 2ME2 caused morphological, immunophenotypic and the supernatant light chain secretion changes typical of differentiation in all the three myeloma cell lines. 2ME2 up-regulated the XBP-1 mRNA expression. XBP-1 and Blimp-1 ASODNs partially inhibited the differentiation of LP-1, CZ-1, NCI-H929 cells induced by 2ME2; whereas pax-5 ASODN did the contrary. After incubated with pax-5 ASODN for 72 hours, LP-1, CZ-1, NCI-H929 cells exhibited characteristic morphologic feature of differentiation. The expression of CD49e was increased statistically (P < 0.05). Light chain secretion in the supernatant was also increased statistically (P < 0.05). After incubation with Blimp-1 ASODN, the level of XBP-1 mRNA was declined, while the level of pax-5 mRNA increased. 2ME2 could induce cell differentiation and...Continue Reading

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Mentioned in this Paper

ITGA5 protein, human
Estradiol, (16 alpha,17 beta)-Isomer
Process of Secretion
Cell Differentiation Process
Integrin alpha5
Supernatant
MHC class II regulatory factor RFX
Cell Secretion
Poly(A) Tail
DNA Helix Destabilizing Proteins

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