Molecular mechanism of transforming growth factor-beta-mediated inhibition of growth arrest and differentiation in a myoblast cell line

Development, Growth & Differentiation
Masanori MurakamiMasataka Nakamura

Abstract

Transforming growth factor-beta (TGF-beta) has been demonstrated to inhibit myogenesis in myoblasts. Here we report that transcriptional upregulation of p21(WAF1/Cip1) and muscle creatinine kinase (MCK) genes in C2C12 cells, which are associated with growth arrest at the G1 phase and representative cell-lineage-specific expression during myogenesis, was inhibited by TGF-beta treatment. C2C12 cells expressed TWIST2, but not TWIST1, which was downregulated in myogenic differentiation in response to low-serum cultures. We further found that TGF-beta prevented differentiation-associated downregulation of TWIST2 transcription, resulting in maintaining TWIST2 mRNA expression as high as that in growing C2C12 cells. Ectopic TWIST2 suppressed the p21 and MCK promoters activated by the exogenous addition of E2A and MyoD and this inhibitory effect of TWIST2 was cancelled by the introduction of short hairpin RNA (shRNA) against TWIST2. On the other hand, TWIST2 shRNA failed to recover endogenous promoter activities from TGF-beta-mediated repression. The results clearly indicate that TGF-beta inhibits G1 arrest and maintains TWIST2 expression in C2C12 cells. Our data however suggest that the TGF-beta signaling pathway may not involve TWIST2...Continue Reading

References

Oct 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S S MurrayE J Murray
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J MassaguéB Nadal-Ginard
Nov 1, 1986·The Journal of Cell Biology·E N OlsonC Wilcox
Mar 15, 1995·Genes & Development·Z F Chen, R R Behringer
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·M B DattoX F Wang
Jan 1, 1997·Nature Genetics·V el GhouzziJ Bonaventure
Feb 22, 1997·Biochimica Et Biophysica Acta·R Maione, P Amati
May 1, 1997·Experimental Cell Research·M HebrokE M Füchtbauer
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·L De AngelisG Cossu
Nov 26, 1999·Journal of Cellular Biochemistry·M S LeeC A Glackin
Sep 8, 2000·The Journal of Biological Chemistry·M ThomasR Kambadur
May 22, 2001·Cellular and Molecular Life Sciences : CMLS·M Kitzmann, A Fernandez
Jan 1, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Xun ShenHarold S Bernstein

❮ Previous
Next ❯

Citations

Nov 26, 2009·Molecules and Cells·Han Seok KohSung Ho Jeon
May 13, 2011·Science Translational Medicine·Tyesha N BurksRonald D Cohn
Nov 29, 2008·Experimental Cell Research·Elske J SchabortCarola U Niesler
Feb 18, 2017·International Journal of Molecular Sciences·Elizabeth A Grunz-BorgmannAlan R Parrish
Sep 10, 2016·American Journal of Physical Medicine & Rehabilitation·Jinyue WangWeidou Feng
Feb 11, 2015·The Journal of Endocrinology·Jonathan M MudryAnna Krook
May 12, 2017·European Journal of Applied Physiology·Lewan ParkerItamar Levinger
Aug 3, 2017·Acta Neuropathologica·Mohini SinghSheila K Singh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
A Zentella, J Massagué
Current Opinion in Genetics & Development
K Walsh, H Perlman
Proceedings of the National Academy of Sciences of the United States of America
Xin PanXue-Min Zhang
© 2021 Meta ULC. All rights reserved